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Titolo:
Characterization of 2-[I-125]-iodomelatonin binding sites in the golden hamster retina by autoradiography
Autore:
Rosenstein, RE; Dubocovich, ML;
Indirizzi:
Univ Buenos Aires, Fac Med, Dept Bioquim Humana, Sch Med,Lab Retinal Neurochem & Expt Ophthalmol, RA-1121 Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina RA-1121 Aires, DF, Argentina Northwestern Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Chicago, IL USA Northwestern Univ Chicago IL USA l Chem & Mol Pharmacol, Chicago, IL USA
Titolo Testata:
BIOLOGICAL RHYTHM RESEARCH
fascicolo: 2, volume: 32, anno: 2001,
pagine: 191 - 200
SICI:
0929-1016(2001)32:2<191:CO2BSI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELATONIN RECEPTORS; GUANINE-NUCLEOTIDES; MAMMALIAN RETINA; LOCALIZATION; 2-IODOMELATONIN; SUBTYPES; DOPAMINE; RHYTHMS;
Keywords:
melatonin receptors; hamster; retina; autoradiography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Rosenstein, RE Univ Buenos Aires, Fac Med, Dept Bioquim Humana, Sch Med,Lab Retinal Neurochem & Expt Ophthalmol, Paraguay 2155,5P, RA-1121 Buenos Aires, DF, Argentina Univ Buenos Aires Paraguay 2155,5P Buenos Aires DF Argentina RA-1121
Citazione:
R.E. Rosenstein e M.L. Dubocovich, "Characterization of 2-[I-125]-iodomelatonin binding sites in the golden hamster retina by autoradiography", BIOL RH RES, 32(2), 2001, pp. 191-200

Abstract

The characterization of 2-[I-125]-iodomelatonin binding sites was performed in the golden hamster retina using in vitro quantitative autoradiography. The specific binding of the radioligand fulfills all the criteria for binding to a receptor site, being stable, reversible, saturable and of high affinity. 2-[I-125]-iodomelatonin labels a single class of sites in the sections of whole eyes as well as in isolated retinas with a similar affinity, whereas the total number of receptors was higher in sections of whole eyes than in isolated retinas. Melatonin and related analogues competed for 2-[I-125]-iodomelatonin binding with the following order of affinities: 2-iodomelatonin > 6-hydroxymelatonin > melatonin > 6-chloromelatonin > > > N-acetyl-5-hydroxytryptamine (NAS) > 5-methoxytryptamine > 5-hydroxytryptamine (serotonin). Micromolar concentrations of GTP and GDP dose-dependently and specifically inhibited agonist binding, suggesting coupling of the binding sitesto a Gi protein. These results suggest the participation of melatonin in the regulation of retinal physiology trough activation of melatonin receptorsubtypes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 02:10:42