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Titolo:
Effect of dyskinetoplastic agents on ultrastructure and oxidative phosphorylation in Crithidia fasciculata
Autore:
Biscardi, AM; Lopez, LM; de Pahn, EM; de Iraldi, AP; Stoppani, AOM;
Indirizzi:
Univ Buenos Aires, Fac Med, Ctr Invest Bioenerget, RA-1121 Buenos Aires, DF, Argentina Univ Buenos Aires Buenos Aires DF Argentina RA-1121 Aires, DF, Argentina Dept Cell Biol & Neurosci, Buenos Aires, DF, Argentina Dept Cell Biol & Neurosci Buenos Aires DF Argentina Aires, DF, Argentina
Titolo Testata:
BIOCELL
fascicolo: 1, volume: 25, anno: 2001,
pagine: 43 - 51
SICI:
0327-9545(200104)25:1<43:EODAOU>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASCITES TUMOR-CELLS; KINETOPLAST DNA; TRYPANOSOMA-CRUZI; ETHIDIUM-BROMIDE; CONSUMING PROCESSES; MINICIRCLE DNA; REPLICATION; INTERCALATION; PROTEINS;
Keywords:
Critihidia fasciculata; trypanosomatids; ethidium bromide; o-naphthoquinones; dyskinetoplasty; oxidative phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Stoppani, AOM Univ Buenos Aires, Fac Med, Ctr Invest Bioenerget, Paraguay 2155,P16, RA-1121 Buenos Aires, DF, Argentina Univ Buenos Aires Paraguay 2155,P16 Buenos Aires DF Argentina RA-1121
Citazione:
A.M. Biscardi et al., "Effect of dyskinetoplastic agents on ultrastructure and oxidative phosphorylation in Crithidia fasciculata", BIOCELL, 25(1), 2001, pp. 43-51

Abstract

Ethidium bromide (EB) is an intercalating agent which binds specifically to the kinetoplast (mitochondrial) DNA (kDNA) of trypanosomatids. Accordingly, EB inhibits DNA replication, thus inducing dyskinetoplasty. Since in eukariotic organisms mitochondrial DNA encodes the genetic information for cytochromes 6, aa(3) and F0F1 ATPase, it seemed of interest to establish whether a similar effect occurs in Crithidia fasciculata, a trypanosomatid used for assay of potential trypanocidal drugs. Culturing of C. fasciculata in the presence of EB inhibited growth and induced dyskinetoplasty, as confirmed by electron microscopy. The kinetoplast of EB-cultured crithidia lost itscharacteristic are shape, it was misplaced in the cell cytoplasm its matrix structure and membrane differentiation were specifically modified. Dyskinetoplasty decreased crithidia respiration and oxidative phosphorylation, asindicated by the lower ATP level, ATP/ADP ratio and adenylate energy charge. The interference of EB with kinetoplastic constituents synthesis was confirmed by the lack of action of EB on crithidia in the stationary phase of growth, that ruled out direct inhibition of oxidative phosphorylation enzymes. The lipophilic o-naphthoquinone beta -lapachone produced structural alterations in kinetoplast membranes, that correlated with inhibition of oxidative phosphorylation. These latter effects involved free radicals since they were prevented by free radical scavengers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:08:50