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Titolo:
Serum factors regulate the expression of the proliferation-related genes alpha 5 integrin and keratin 1, but not keratin 10, in HaCaT keratinocytes
Autore:
Pivarcsi, A; Szell, M; Kemeny, L; Dobozy, A; Bata-Csorgo, Z;
Indirizzi:
Univ Szeged, Dept Dermatol, H-6720 Szeged, Hungary Univ Szeged Szeged Hungary H-6720 Dept Dermatol, H-6720 Szeged, Hungary Hungarian Acad Sci, Dermatol Res Grp, Szeged, Hungary Hungarian Acad Sci Szeged Hungary ci, Dermatol Res Grp, Szeged, Hungary
Titolo Testata:
ARCHIVES OF DERMATOLOGICAL RESEARCH
fascicolo: 4, volume: 293, anno: 2001,
pagine: 206 - 213
SICI:
0340-3696(200104)293:4<206:SFRTEO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN EPIDERMAL-KERATINOCYTES; CULTURED HUMAN KERATINOCYTES; TERMINAL DIFFERENTIATION; FIBRONECTIN RECEPTOR; EXTRACELLULAR-MATRIX; PSORIATIC SKIN; CELL-SURFACE; GROWTH; PHENOTYPE; ADHESION;
Keywords:
HaCaT keratinocytes; proliferation; differentiation; alpha 5 integrin; keratins 1 and 10;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Pivarcsi, A Univ Szeged, Dept Dermatol, Koranyi Fasor 6, H-6720 Szeged, Hungary Univ Szeged Koranyi Fasor 6 Szeged Hungary H-6720 ed, Hungary
Citazione:
A. Pivarcsi et al., "Serum factors regulate the expression of the proliferation-related genes alpha 5 integrin and keratin 1, but not keratin 10, in HaCaT keratinocytes", ARCH DERM R, 293(4), 2001, pp. 206-213

Abstract

In the highly coordinated programme of gene expression during keratinocyteproliferation and differentiation, alpha5 integrin and keratins 1 and 10 (K1/K10) may play important regulatory roles. We were interested in seeing whether, in continuously growing, immortalized HaCaT keratinocytes, similar to normal keratinocytes, the expression of alpha5 integrin and K1/K10 was related to cell proliferation and differentiation. After release from cell quiescence the expression of alpha5 integrin, both at the mRNA and protein levels, was upregulated in the cells. At the same time, K1/K10 mRNA and protein expression decreased dramatically, while the mRNA for D1 cyclin became detectable, and the cells became highly proliferative. These findings indicate that alpha5 integrin and K1/K10 are involved in the regulation of HaCaTproliferation and differentiation, as in normal keratinocytes. However, HaCaT cells are different from normal keratinocytes in their ability to lose K1/K10 expression. There is no evidence that the expression of K1/K10 can be reversed in normal keratinocytes. This ability of dedifferentiation mightbe a unique feature of HaCaT cells and may be a key component of their immortalized nature. We also found that serum factors regulate mRNA expressionof alpha5 integrin and K1, but not of K10, in HaCaT cells. This information could be relevant to the understanding of normal epidermal differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:18:20