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Titolo:
Dissemination of catabolic plasmids among desiccation-tolerant bacteria insail microcosms
Autore:
Weekers, F; Rodriguez, C; Jacques, P; Mergeay, M; Thonart, P;
Indirizzi:
Univ Liege, Walloon Ctr Ind Biol, B-4000 Liege, Belgium Univ Liege LiegeBelgium B-4000 loon Ctr Ind Biol, B-4000 Liege, Belgium Agr Univ Gembloux, Walloon Ctr Ind Biol, B-5030 Gembloux, Belgium Agr UnivGembloux Gembloux Belgium B-5030 Biol, B-5030 Gembloux, Belgium Belgian Nucl Res Ctr, B-2400 Mol, Belgium Belgian Nucl Res Ctr Mol Belgium B-2400 ucl Res Ctr, B-2400 Mol, Belgium
Titolo Testata:
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
, volume: 91-3, anno: 2001,
pagine: 219 - 232
SICI:
0273-2289(200121)91-3:<219:DOCPAD>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHODOCOCCUS-ERYTHROPOLIS BD2; INDIGENOUS SOIL BACTERIA; HORIZONTAL TRANSFER; LINEAR PLASMID; GENE-TRANSFER; DEGRADATION; ISOPROPYLBENZENE; BIOAUGMENTATION; IDENTIFICATION; BIOREMEDIATION;
Keywords:
bioaugmentation; drought tolerance; conjugation; plasmid dissemination; microcosm; isopropylbenzene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Weekers, F Univ Liege, Walloon Ctr Ind Biol, B40, B-4000 Liege, Belgium Univ Liege B40 Liege Belgium B-4000 B40, B-4000 Liege, Belgium
Citazione:
F. Weekers et al., "Dissemination of catabolic plasmids among desiccation-tolerant bacteria insail microcosms", APPL BIOC B, 91-3, 2001, pp. 219-232

Abstract

The dissemination of catabolic plasmids was compared to bioaugmentation bystrain inoculation in microcosm experiments. When Rhodococcus erythropolisstrain T902, bearing a plasmid with trichloroethene and isopropylbenzene degradation pathways, was used as the inoculum, no transconjugant was isolated but the strain remained in the soil. This plasmid had a narrow host range. Pseudomonas putida strain C8S3 was used as the inoculum in a second approach. It bore a broad host range conjugative plasmid harboring a natural transposon, RP4::Tn4371, responsible for biphenyl and 4-chlorobiphenyl degradation pathways. The inoculating population slowly decreased from its original level (10(6) colony-forming units [CFU]/g of dry soil) to approx 3 x 10(2) CFU/g of dry soil after 3 wk. Transconjugant populations degrading biphenyl appeared in constant humidity soil (up to 2 x 10(3) CFU/g) and desiccating soil (up to 10(4) CFU/g). The feasibility of plasmid dissemination as abioaugmentation technique was demonstrated in desiccating soils. The ecologic significance of desiccation in bioaugmentation was demonstrated: it upset the microbial ecology and the development of transconjugants.

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Documento generato il 02/04/20 alle ore 17:03:55