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Titolo:
Derivatization and fluorescence detection of amino acids and peptides with9-fluorenylmethyl chloroformate on the surface of a solid adsorbent
Autore:
Shangguan, DH; Zhao, YX; Han, HW; Zhao, R; Liu, GQ;
Indirizzi:
Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China Chinese Acad Sci Beijing Peoples R China 100080 100080, Peoples R China
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 9, volume: 73, anno: 2001,
pagine: 2054 - 2057
SICI:
0003-2700(20010501)73:9<2054:DAFDOA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; PHASE DERIVATIZATION; PRECOLUMN DERIVATIZATION; URINE; AMPHETAMINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, GQ Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China Chinese Acad Sci Beijing Peoples R China 100080 Peoples R China
Citazione:
D.H. Shangguan et al., "Derivatization and fluorescence detection of amino acids and peptides with9-fluorenylmethyl chloroformate on the surface of a solid adsorbent", ANALYT CHEM, 73(9), 2001, pp. 2054-2057

Abstract

An approach that exploits the surface of a solid adsorbent is proposed forprecolumn FMOC derivatization of amino acids and peptides. Amino acids (Ser, Glu, GABA, Val, Phe, Lys) and two neuropeptides (substance P and Leuenkephalin) were adsorbed on alkaline silica gel cartridges. After drying, theywere reacted with 9-fluorenylmethyl chloroformate: (FMOC-Cl) in toluene. After washing off the excess FMOC-Cl with ethyl acetate, the derivatives were eluted with aqueous eluant. The eluates were separated and detected by means of HPLC with fluorescence detection. Compared with the traditional derivatization in the liquid phase, the extent of formation of byproducts of FMOC-Cl with water was greatly decreased, and the excess FMOC-Cl was eliminated completely.

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Documento generato il 03/04/20 alle ore 04:23:48