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Titolo:
Gas chromatography/mass spectrometry method to quantify blood hydroxycitrate concentration
Autore:
Loe, YCC; Bergeron, N; Rodriguez, N; Schwarz, JM;
Indirizzi:
Univ Calif Berkeley, Dept Nutr Sci, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 utr Sci, Berkeley, CA 94720 USA
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 1, volume: 292, anno: 2001,
pagine: 148 - 154
SICI:
0003-2697(20010501)292:1<148:GCSMTQ>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FATTY ACID SYNTHESIS; OXIDATION;
Keywords:
hydroxycitrate; Garcinia cambogia; dietary supplement; GC/MS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Schwarz, JM Univ Calif Berkeley, Dept Nutr Sci, 119 Morgan Hall, Berkeley,CA 94720 USA Univ Calif Berkeley 119 Morgan Hall Berkeley CA USA 94720 USA
Citazione:
Y.C.C. Loe et al., "Gas chromatography/mass spectrometry method to quantify blood hydroxycitrate concentration", ANALYT BIOC, 292(1), 2001, pp. 148-154

Abstract

Hydroxycitrate (HCA), a popular dietary supplement for weight loss, is a competitive inhibitor of ATP-citrate lyase, an extramitochondrial enzyme involved in the initial steps of de novo lipogenesis (DNL), Although animal studies have shown that HCA effectively inhibits DNL and induces weight loss,these findings have not been consistent in humans. This raises the possibility that the bioavailability of RCA may differ among species. We developeda new GC/MS method to measure HCA levels in blood, using [U-C-13]citrate (CA*) as internal standard to account for losses associated with the isolation, derivatization, and measurement of HCA. HCA and CA* were derivatized with BSTFA + 10% TMCS and analyzed using PCI/GC/MS (CA*, m/z 471; and HCA, m/z 553). The plasma HCA concentration was measured over a 3.5-h period in four subjects having ingested 2 g of HCA, Their plasma HCA concentration ranged from 0.8 to 8.4 mug/ml 30 min and 2 h after ingestion, respectively. These results demonstrate that when taken acutely, HCA is absorbed, yet present in small quantities in human plasma. This simple method requiring minimalsample preparation is able to measure trace amounts of HCA with accuracy and precision. (C) 2001 Academic Press.

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Documento generato il 30/09/20 alle ore 09:43:36