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Titolo:
Mammalian heat shock p70 and histone H4 transcripts, which derive from naturally intronless genes, are immune to nonsense-mediated decay
Autore:
Maquat, LE; Li, XJ;
Indirizzi:
Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 & Biophys, Rochester, NY 14642 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 3, volume: 7, anno: 2001,
pagine: 445 - 456
SICI:
1355-8382(200103)7:3<445:MHSPAH>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA SURVEILLANCE; INITIATION-FACTOR 4E; DEPENDENT GLUTATHIONE-PEROXIDASE-1; TRANSLATION TERMINATION; CELLS; PROTEIN; ABUNDANCE; EXPRESSION; SEQUENCES; CYTOPLASM;
Keywords:
heat shock gene; histone gene; immunity to nonsense-mediated mRNA decay; naturally intronless genes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Maquat, LE Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, 601 Elmwood Ave, Rochester, NY 14642 USA Univ Rochester 601 Elmwood Ave Rochester NY USA 14642 14642 USA
Citazione:
L.E. Maquat e X.J. Li, "Mammalian heat shock p70 and histone H4 transcripts, which derive from naturally intronless genes, are immune to nonsense-mediated decay", RNA, 7(3), 2001, pp. 445-456

Abstract

Nonsense-mediated decay (NMD), also called mRNA surveillance, is an evolutionarily conserved pathway that degrades mRNAs that prematurely terminate translation. To date, the pathway in mammalian cells has been shown to depend on the presence of a cis-acting destabilizing element that usually consists of an exon-exon junction generated by the process of pre-mRNA splicing. Whether or not mRNAs that derive from naturally intronless genes, that is, mRNAs not formed by the process of splicing, are also subject to NMD has yet to be investigated. The possibility of NMD is certainly reasonable considering that mRNAs of Saccharomyces cerevisiae are subject to NMD even thoughmost derive from naturally intronless genes. In fact, mRNAs of S. cerevisiae generally harbor a loosely defined splicing-independent destabilizing element that has been proposed to function in NMD analogously to the spliced exon-exon junction of mammalian mRNAs. Here, we demonstrate that nonsense codons introduced into naturally intronless genes encoding mouse heat shock protein 70 or human histone H4 fail to elicit NMD, Failure is most likely because each mRNA lacks a cis-acting destabilizing element, because insertion of a spliceable intron a sufficient distance downstream of a nonsense codon within either gene is sufficient to elicit NMD,

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 04:27:03