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Titolo:
Inhibition of human and ovine acrosomal enzymes by tannic acid in vitro
Autore:
Taitzoglou, IA; Tsantarliotou, M; Zervos, I; Kouretas, D; Kokolis, NA;
Indirizzi:
Univ Thessaloniki, Fac Vet Med, Dept Physiol, GR-43100 Karditsa, Greece Univ Thessaloniki Karditsa Greece GR-43100 ol, GR-43100 Karditsa, Greece Aristotelian Univ Salonika, Fac Med Vet, Dept Physiol, GR-54006 Salonika, Greece Aristotelian Univ Salonika Salonika Greece GR-54006 006 Salonika, Greece Univ Thessaly, Sch Agr Sci, Dept Physiol, Volos, Greece Univ Thessaly Volos Greece ly, Sch Agr Sci, Dept Physiol, Volos, Greece
Titolo Testata:
REPRODUCTION
fascicolo: 1, volume: 121, anno: 2001,
pagine: 131 - 137
SICI:
1470-1626(200101)121:1<131:IOHAOA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR INHIBITION; HUMAN-SPERM PENETRATION; HUMAN-SPERMATOZOA; ZONA-PELLUCIDA; SEMINAL PLASMA; PROTEINASE-INHIBITORS; HYDROLYTIC ENZYMES; ACROSIN ACTIVITY; FLAVONOIDS; GOSSYPOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Kokolis, NA Univ Thessaloniki, Fac Vet Med, Dept Physiol, GR-43100 Karditsa, Greece Univ Thessaloniki Karditsa Greece GR-43100 Karditsa, Greece
Citazione:
I.A. Taitzoglou et al., "Inhibition of human and ovine acrosomal enzymes by tannic acid in vitro", REPRODUCT, 121(1), 2001, pp. 131-137

Abstract

The effect of tannic acid, a common flavonoid, on the acrosin and plasminogen activator activity and plasmin activity of human and ram spermatozoa was evaluated. Acrosin and plasminogen activator activity were determined by spectrophotometry using the chromogenic substrates N-alpha -benzoyl-DL-arginine para-nitroanilide-HCl (BAPNA) and H-D-valy-L-leucyl-L-lysine-p-nitroanilide -2HCI (S-2251), respectively. In extracts from both human and ovine acrosomes, the activities of acrosin and plasminogen activators were susceptible to tannic acid inhibition. The inhibitory effect of tannic acid was observed at concentrations > 50 mu mol l(-1) in a dose-dependent manner, in additional experiments, low concentrations of tannic acid significantly inhibited tissue-type plasminogen activator, urokinase-type plasminogen activator and plasmin activity in a concentration-dependent manner over the range 0.25-200 mu mol l(-1). Tannic acid reduced the motility of ram spermatozoa at a concentration of 1000 mu mol l(-1) after 2 and 3 h co-incubation with spermatozoa. The motility of human spermatozoa remained unchanged over the range 0.1-1000 mu mol tannic acid l(-1) during 3 h co-incubation. These results indicate that tannic acid inhibited the activity of both acrosin and plasminogen activator and indicates a possible mechanism by which flavonoidsexert their antifertility effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 21:59:55