Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Maternal-conceptus signalling during early pregnancy in mares: oestrogen and insulin-like growth factor 1
Autore:
Walters, KW; Roser, JF; Anderson, GB;
Indirizzi:
Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 s, Dept Anim Sci, Davis, CA 95616 USA
Titolo Testata:
REPRODUCTION
fascicolo: 2, volume: 121, anno: 2001,
pagine: 331 - 338
SICI:
1470-1626(200102)121:2<331:MSDEPI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
THECA-INTERSTITIAL CELLS; ELONGATING PORCINE BLASTOCYSTS; MESSENGER-RIBONUCLEIC-ACID; RAT GRANULOSA-CELLS; FACTOR-I EXPRESSION; ESTROGEN-RECEPTOR; GENE-EXPRESSION; SOMATOMEDIN-C; CYTOCHROME-P450 AROMATASE; EQUINE EMBRYO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Walters, KW Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 Sci, Davis, CA 95616 USA
Citazione:
K.W. Walters et al., "Maternal-conceptus signalling during early pregnancy in mares: oestrogen and insulin-like growth factor 1", REPRODUCT, 121(2), 2001, pp. 331-338

Abstract

Embryonic production of oestrogen is thought to play an important role in conceptus-maternal signalling during early pregnancy in mares, and may be regulated in an autocrine or paracrine fashion by insulin-like growth factorI (IGF-I). In this study, the hypothesis that IGF-I stimulates embryonic oestrogen synthesis, which in turn stimulates uterine IGF-I secretion was tested. Specific sources of IGF-I in the uterine lumen were characterized. Preimplantation embryos, uterine biopsies, and uterine flush fluids were collected on day 13 of pregnancy. Embryos were cultured whole for 24 h, or dispersed and incubated in serum-free culture medium supplemented with androstenedione or testosterone (0-10 mug ml(-1)) and IGF-I (0-100 ng ml(-1)). Oestrogen synthesis was increased by addition of androgen, but there was no dose-dependent effect of IGF-I. Endometrial explants were cultured for 24, 48 and 72 h in serum-free medium supplemented with oestradiol. IGF-I was measured by radioimmunoassay in embryo-conditioned medium, explant culture medium, blastocoelic fluid, concentrated (x 100) uterine flush fluid and endometrial-tissue homogenate. Both the embryo and endometrium produced significant quantities of IGF-I, indicating a role for this growth factor in autocrine-paracrine signalling during early pregnancy. However, secretion of IGF-I by endometrial explants was not modulated by oestrogen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 04:16:49