Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of genistein on the periovulatory expression of messenger ribonucleic acid for matrix metalloproteinases and tissue inhibitors of metalloproteinases in the rat ovary
Autore:
Komar, CM; Matousek, M; Mitsube, K; Mikuni, M; Brannstrom, M; Curry, TE;
Indirizzi:
Univ Kentucky, Chandler Med Ctr, Dept Obstet & Gynecol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 t & Gynecol, Lexington, KY 40536 USA Gothenburg Univ, Sahlgrens Hosp, Dept Obstet & Gynecol, S-41345 Gothenburg, Sweden Gothenburg Univ Gothenburg Sweden S-41345 ol, S-41345 Gothenburg, Sweden
Titolo Testata:
REPRODUCTION
fascicolo: 2, volume: 121, anno: 2001,
pagine: 259 - 265
SICI:
1470-1626(200102)121:2<259:EOGOTP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTAGLANDIN ENDOPEROXIDE SYNTHASE-2; GONADOTROPIN-INDUCED OVULATION; LUTEINIZING-HORMONE; GRANULOSA-CELLS; TYROSINE KINASE; GELATINASE-A; ARTICULAR CHONDROCYTES; SIGNALING PATHWAYS; PROTEIN-SYNTHESIS; PHOSPHOLIPASE-C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Komar, CM Univ Kentucky, Chandler Med Ctr, Dept Obstet & Gynecol, 800 RoseSt,Room MS 331, Lexington, KY 40536 USA Univ Kentucky 800 Rose St,Room MS 331 Lexington KY USA 40536 USA
Citazione:
C.M. Komar et al., "Effects of genistein on the periovulatory expression of messenger ribonucleic acid for matrix metalloproteinases and tissue inhibitors of metalloproteinases in the rat ovary", REPRODUCT, 121(2), 2001, pp. 259-265

Abstract

The matrix metalloproteinases (MMPs) play critical roles in the ovulatory process. Their expression and activity, together with those of the endogenous tissue inhibitors of metalloproteinases (TIMPs), are stimulated by LH. The LH surge initiates a cascade of events resulting in ovulation and formation of the corpus luteum via activation of protein kinases A and C, as wellas tyrosine kinases. In vitro perfused rat ovaries were untreated, or treated with LH (0.2 mug ml(-1)) plus 0.2 mmol 3-isobutyl-1-methylxanthine I-1 with 0, 10 or 100 mu mol genistein I-1 (an inhibitor of tyrosine kinases) to assess whether tyrosine kinases are mediators of the LH-stimulated increase in ovarian expression of the MMPs and TIMPs. After 10 h of perfusion, ovaries were collected and frozen until RNA isolation. Northern and RNase protection analyses were used to measure mRNA encoding collagenase 3, gelatinases A and B, and TIMPs-1, -2 and -3. Treatment with LH plus 3-isobutyl-1-methylxanthine resulted in a two- and fivefold increase in mRNA encoding collagenase 3 and TIMP-1, respectively (P < 0.05), Treatment with 100 mu mol genistein I-1 blocked the LH-stimulated increase in collagenase 3 (0.012 +/- 0.002 versus 0.028 +/- 0.005 relative units for 100 mu mol genistein I-1 versus LH; P < 0.05), whereas neither dose of genistein affected LH-induced TIMP-1 expression. LH alone or with genistein did not alter the expression of mRNA encoding TIMP-2 and TIMP-3, or mRNA encoding gelatinases A and B. These data indicate that tyrosine kinases play a role in the LH-induced tissue remodelling required for ovulation by mediating the LH-stimulated expression of collagenase 3.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:01:39