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Titolo:
In vitro maturation, fertilization and embryo development after ultrarapidfreezing of immature human oocytes
Autore:
Wu, J; Zhang, L; Wang, X;
Indirizzi:
Beijing Med Univ, Dept Obstet & Gynecol, Clin Med Coll 3, Beijing 100083, Peoples R China Beijing Med Univ Beijing Peoples R China 100083 100083, Peoples R China
Titolo Testata:
REPRODUCTION
fascicolo: 3, volume: 121, anno: 2001,
pagine: 389 - 393
SICI:
1470-1626(200103)121:3<389:IVMFAE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROPHASE-I OOCYTES; MOUSE OOCYTES; INVITRO FERTILIZATION; CRYOPRESERVATION; EGGS; PREGNANCY; CAPACITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Wu, J Univ Utah, Sch Med, Div Urol, 50 Med Dr, Salt Lake City, UT 84132 USA Univ Utah 50 Med Dr Salt Lake City UT USA 84132 e City, UT 84132 USA
Citazione:
J. Wu et al., "In vitro maturation, fertilization and embryo development after ultrarapidfreezing of immature human oocytes", REPRODUCT, 121(3), 2001, pp. 389-393

Abstract

The purpose of this study was to determine the rates of maturation, fertilization and embryo development of ultrarapidly frozen immature oocytes (immature cumulus-oocyte complexes; COCs) obtained from antral follicles in ovaries of patients with chocolate ovarian cysts. The COCs were cryopreserved by a vitrification method using 5.5 mol ethylene glycol l(-1) plus 1.0 mol sucrose l(-1) in Dulbecco's PBS (DPBS). The survival, maturation and fertilization rates, and the percentage of embryos developing to the two-cell stage were 59, 64, 70 and 71 %, respectively. No significant differences were noted in the rates of maturation, fertilization and embryo development between control and cryopreserved oocytes. Two embryos that developed from cryopreserved oocytes of the oocyte donor programme were selected for transfer into the uterus of a recipient with premature ovarian failure, after the recipient had received steroid replacement. A biochemical pregnancy occurred in the recipient after embryo transfer. These results indicate that immature oocytes can survive after cryopreservation and subsequently can be cultured to mature oocytes that are capable of undergoing fertilization in vitro and developing into embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 21:00:51