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Titolo:
Secretion of matrix metalloproteinases 2 and 9 and tissue inhibitor of metalloproteinases into follicular fluid during follicle development in equineovaries
Autore:
Riley, SC; Gibson, AH; Leask, R; Mauchline, DJW; Pedersen, HG; Watson, ED;
Indirizzi:
Univ Edinburgh, Dept Reprod & Dev Sci, Obstet & Gynaecol Sect, Edinburgh EH3 9ET, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH3 9ET Midlothian, Scotland Univ Edinburgh, Dept Vet Clin Studies, Edinburgh EH8 9YL, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH8 9YL Midlothian, Scotland Univ Edinburgh, Ctr Reprod Biol, Edinburgh EH3 9ET, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH3 9ET Midlothian, Scotland
Titolo Testata:
REPRODUCTION
fascicolo: 4, volume: 121, anno: 2001,
pagine: 553 - 560
SICI:
1470-1626(200104)121:4<553:SOMM2A>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
STROMAL CELLS; IN-VITRO; LOCALIZATION; EXPRESSION; OVULATION; RAT; MATRIX-METALLOPROTEINASE-9; IDENTIFICATION; PROGESTERONE; GELATINASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Riley, SC Univ Edinburgh, Dept Reprod & Dev Sci, Obstet & Gynaecol Sect, 37 ChalmersSt, Edinburgh EH3 9ET, Midlothian, Scotland Univ Edinburgh 37 Chalmers St Edinburgh Midlothian Scotland EH3 9ET
Citazione:
S.C. Riley et al., "Secretion of matrix metalloproteinases 2 and 9 and tissue inhibitor of metalloproteinases into follicular fluid during follicle development in equineovaries", REPRODUCT, 121(4), 2001, pp. 553-560

Abstract

Extensive tissue remodelling is required in equine ovaries for follicle growth and development and also migration of the follicle to the ovulatory fossa, where ovulation occurs. The mechanisms for these processes are largelyunexplored. Matrix metalloproteinases (MMPs) and their endogenous tissue inhibitors (TIMPs) are important for control of breakdown of extracellular matrix during tissue remodelling. The aims of this study were to determine the pattern and sites of secretion of the gelatinases MMP-2 and -9 and TIMPsinto follicular fluid during follicle development in mare ovaries. The predominant gelatinase detected in follicular fluid was MMP-2, which was present in similar amounts throughout follicular development, as demonstrated byzymography. MMP-9 was also present in follicular fluid and secretion increased significantly (P < 0.05) with development of follicles from < 10 mm to11-20 mm in diameter. Follicular fluid also contained TIMP-1, TIMP-2, unglycosylated and glycosylated TIMP-3, and TIMP-4, as shown by reverse zymography. The abundance of TIMPs remained largely unchanged during follicle development. MMP-2 and -9 were localized by immunohistochemistry to stromal cells and granulosa and theca cells. TIMP-1, -2, -3 and -4 were present in granulosa and theca cells of the follicle and in stromal cells and also associated with extracellular matrix of the ovarian stromal tissue. The MMPs and TIMPs are likely to be involved in the regulation of the breakdown of extracellular matrix during tissue remodelling for follicle development and migration to the ovulation fossa in mares.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:38:06