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Titolo:
An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures
Autore:
Rens, W; Yang, F; Welch, G; Revell, S; OBrien, PCM; Solanky, N; Johnson, LA; Smith, MAF;
Indirizzi:
Univ Cambridge, Dept Clin Vet Med, Ctr Vet Sci, Vet Cytogenet Grp, Cambridge CB3 0ES, England Univ Cambridge Cambridge England CB3 0ES Grp, Cambridge CB3 0ES, England USDA ARS, Beltsville Agr Res Ctr, Beltsville Agr Res Ctr E, GGPL, Beltsville, MD 20705 USA USDA ARS Beltsville MD USA 20705 es Ctr E, GGPL, Beltsville, MD 20705 USA Genus Plc, Ruthin, Clywd, Wales Genus Plc Ruthin Clywd WalesGenus Plc, Ruthin, Clywd, Wales
Titolo Testata:
REPRODUCTION
fascicolo: 4, volume: 121, anno: 2001,
pagine: 541 - 546
SICI:
1470-1626(200104)121:4<541:AXPSAS>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMOSOME-BEARING SPERM; IN-VITRO FERTILIZATION; SITU HYBRIDIZATION; FLOW-CYTOMETRY; BOVINE SPERM; SPERMATOZOA; DNA; PRESELECTION; SEMEN; DECONDENSATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Rens, W Univ Cambridge, Dept Clin Vet Med, Ctr Vet Sci, Vet Cytogenet Grp,Madingley Rd, Cambridge CB3 0ES, England Univ Cambridge Madingley Rd Cambridge England CB3 0ES ES, England
Citazione:
W. Rens et al., "An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures", REPRODUCT, 121(4), 2001, pp. 541-546

Abstract

X and Y chromosome paints were developed from sorted yak chromosomes for sexing cattle spermatozoa. Clear hybridization signals were obtained for every spermatozoon using a modified sperm decondensation protocol and fluorescence in situ hybridization (FISH). The procedure was evaluated using the established Beltsville sperm sexing technology, which separates spermatozoa by flow cytometry into X- and Y-bearing fractions. Close agreement was foundbetween the assessment of sperm separation by flow cytometry and by FISH with the X-Y paint set. The FISH method is a simple, reliable and robust procedure for assessing the effectiveness of separation of X and Y spermatozoa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:56:48