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Titolo:
Insertion of polydeoxyadenosine-rich sequences into an intergenic region increases transcription in Chlamydomonas reinhardtii chloroplasts
Autore:
Lisitsky, I; Rott, R; Schuster, G;
Indirizzi:
Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-32000 L-32000 Haifa, Israel
Titolo Testata:
PLANTA
fascicolo: 5-6, volume: 212, anno: 2001,
pagine: 851 - 857
SICI:
0032-0935(200104)212:5-6<851:IOPSIA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA ACCUMULATION; 3' INVERTED REPEATS; GENE-EXPRESSION; 3'-UNTRANSLATED REGIONS; TOBACCO PLASTIDS; PSBB OPERON; DNA; PROMOTER; POLYMERASE; MATURATION;
Keywords:
transcription (chloroplastic); chloroplast; polydeoxyadenosine; chloroplast transformation; Chlamydomonas (chloroplast transformation);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Schuster, G Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-32000 a, Israel
Citazione:
I. Lisitsky et al., "Insertion of polydeoxyadenosine-rich sequences into an intergenic region increases transcription in Chlamydomonas reinhardtii chloroplasts", PLANTA, 212(5-6), 2001, pp. 851-857

Abstract

In this study, chloroplast transformation in Chlamydomonas reinhardtii wasused to insert a tract of polydeoxyadenosine, which is known to influence DNA structure and transcription in other systems, between the 3' end of theatpB gene, encoding the beta -subunit of the chloroplast ATP synthase, anda downstream chimeric gene, aadA, encoding antibiotic resistance. Run-on transcription and RNA analyses revealed that in cells containing (dA)(40) and (dAAAGGG)(8), aadA was transcribed at a higher rate, and its RNA accumulated to a relatively high level. It is concluded that poly(dA/dT) can function in the chloroplast as a transcription enhancer element. Therefore, the insertion of poly(dA/dT) sequence into the intergenic region of a multicistronic transcription unit may modulate gene expression at the transcriptionallevel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 16:08:14