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Titolo:
Genetic relationships and isozyme variability in the Heterodera avenae complex determined by isoelectrofocusing
Autore:
Andres, MF; Romero, MD; Montes, MJ; Delibes, A;
Indirizzi:
CSIC, Dept Protecc Vegetal, Ctr Ciencias Medioambientales, E-28006 Madrid,Spain CSIC Madrid Spain E-28006 iencias Medioambientales, E-28006 Madrid,Spain Escuela Tecn Super Ingn Agron, Dept Biotecnol, Madrid 28040, Spain EscuelaTecn Super Ingn Agron Madrid Spain 28040 ol, Madrid 28040, Spain
Titolo Testata:
PLANT PATHOLOGY
fascicolo: 2, volume: 50, anno: 2001,
pagine: 270 - 279
SICI:
0032-0862(200104)50:2<270:GRAIVI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREAL CYST NEMATODES; GEL-ELECTROPHORESIS; INTRASPECIFIC DIVERSITY; PROTEIN-PATTERNS; IDENTIFICATION; MELOIDOGYNE; PHENOTYPES; POPULATIONS; DNA; ESTERASE;
Keywords:
cereal cyst nematode; Heterodera avenae complex; isoelectrofocusing; isozymes; taxonomy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Andres, MF CSIC, Dept Protecc Vegetal, Ctr Ciencias Medioambientales, Serrano 115 Dpdo, E-28006 Madrid, Spain CSIC Serrano 115 Dpdo Madrid Spain E-28006 28006 Madrid, Spain
Citazione:
M.F. Andres et al., "Genetic relationships and isozyme variability in the Heterodera avenae complex determined by isoelectrofocusing", PLANT PATH, 50(2), 2001, pp. 270-279

Abstract

Isozyme variability was assessed among the principal species of the cerealcyst nematode complex to complete and enhance the information provided by classical nematode systematics, in order to clarify inter- and intraspecific relationships within this complex. Twenty populations of cereal cyst nematodes (Heterodera avenae, H. filipjevi, H. latipons and H. mani) were compared by means of five different isoenzymatic systems (esterase, malate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase and superoxide dismutase) using isoelectrofocusing (IEF) on the electrophoretic separation. The results are in agreement with previous morphological and biochemical characterizations, which established genetic diversity between the Gotland strain and H. avenae and identified the Gotland strain with H. filipjevi. Populations from Israel, all included in the H. avenae group, exhibited well-definedintraspecific dissimilarity. The highest degree of polymorphism was found in the H. avenae group for all five enzymatic systems studied. The H. mani population was also included in the H. avenae group by these isozyme analyses. Malate dehydrogenase, phosphoglucoisomerase and phosphoglucomutase isozymes, fractionated for the first time by IEF in the cereal cyst nematode complex, displayed a higher level of polymorphism than using conventional electrophoresis. isoelectric focusing has proved to be a useful tool for detecting genetic diversity within and among species of the cereal cyst nematodecomplex and for taxonomic purposes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 06:47:23