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Titolo:
Gene expression of the taurine transporter and taurine biosynthetic enzymes in rat kidney after antidiuresis and salt loading
Autore:
Bitoun, M; Levillain, O; Tappaz, M;
Indirizzi:
Fac Med RTH Laennec, INSERM, U 433, F-69372 Lyon 08, France Fac Med RTH Laennec Lyon France 08 NSERM, U 433, F-69372 Lyon 08, France Fac Med RTH Laennec, INSERM, U 499, F-69372 Lyon, France Fac Med RTH Laennec Lyon France F-69372 ERM, U 499, F-69372 Lyon, France
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 1, volume: 442, anno: 2001,
pagine: 87 - 95
SICI:
0031-6768(200104)442:1<87:GEOTTT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDULLARY ORGANIC OSMOLYTES; COTRANSPORTER MESSENGER-RNA; ALDOSE REDUCTASE GENE; INOSITOL COTRANSPORTER; STIMULATES TRANSCRIPTION; SORBITOL DEHYDROGENASE; MOLECULAR-CLONING; NA+/MYO-INOSITOL; RAPID REGULATION; MDCK CELLS;
Keywords:
aldose reductase; betaine transporter; cysteine dioxygenase; cysteine sulfinate decarboxylase; myo-inositol transporter; taurine transporter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Tappaz, M Fac Med RTH Laennec, INSERM, U 433, Rue Guillaume Paradin, F-69372 Lyon 08, France Fac Med RTH Laennec Rue Guillaume Paradin Lyon France 08 France
Citazione:
M. Bitoun et al., "Gene expression of the taurine transporter and taurine biosynthetic enzymes in rat kidney after antidiuresis and salt loading", PFLUG ARCH, 442(1), 2001, pp. 87-95

Abstract

Taurine is thought to be an osmolyte in the kidney medulla. We have investigated the gene expression of the taurine transporter (TauT) and the enzymes of taurine biosynthesis, cysteine dioxygenase (CDO) and cysteine sulfinate decarboxylase (CSD). We achieved this by measuring their mRNA levels using reverse transcriptase polymerase chain reaction (RT-PCR) in five kidney regions of rats in various hydration states; namely, normal hydration, after2 days of antidiuresis following chronic diuresis and finally after acute salt loading. The mRNA levels of the well-established tonicity-sensitive genes coding for the aldose reductase (AR), the sodium myo-inositol transporter (SMIT) and the betaine transporter (BGT1) were also determined for the sake of comparison. In normally hydrated rats, TauT-, CDO-, and CSD-mRNA were enriched in the outer stripe of the outer medulla (OS). Following antidiuresis, the mRNA levels of TauT, CDO, CSD, SMIT, BGT1 and AR were all similarly increased in the papilla when compared with levels in rats submitted toa chronic diuresis. After acute salt loading, the mRNA level of TauT, likethat of SMIT and BGT1, was overexpressed in OS whereas the mRNA levels of CDO and CSD remained unchanged. Like SMIT, BGT1 and AR genes, TauT, CDO andCSD genes appear to be tonicity-sensitive genes which can be activated in vivo by hyper-tonicity in the rat kidney. However, tonicity-induced activation of the TauT gene is more sensitive than that of CDO and CSD genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:07:09