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Titolo:
Differential expression of K-V and K-Ca channels in vascular smooth musclecells during 1-day culture
Autore:
Tang, GH; Wang, R;
Indirizzi:
Univ Saskatchewan, Dept Physiol, Saskatoon, SK S7N 5E5, Canada Univ Saskatchewan Saskatoon SK Canada S7N 5E5 skatoon, SK S7N 5E5, Canada
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 1, volume: 442, anno: 2001,
pagine: 124 - 135
SICI:
0031-6768(200104)442:1<124:DEOKAK>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT TAIL ARTERY; POTASSIUM CHANNELS; CORONARY MYOCYTES; CURRENTS; PULMONARY; MODULATION;
Keywords:
4-aminopyridine; Ca2+-activated K+ channel; iberiotoxin; patch-clamp; peripheral artery; primary culture; smooth muscle cells; voltage-dependent K+ channel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, R Univ Saskatchewan, Dept Physiol, 107 Wiggins Rd, Saskatoon, SK S7N5E5, Canada Univ Saskatchewan 107 Wiggins Rd Saskatoon SK Canada S7N 5E5 anada
Citazione:
G.H. Tang e R. Wang, "Differential expression of K-V and K-Ca channels in vascular smooth musclecells during 1-day culture", PFLUG ARCH, 442(1), 2001, pp. 124-135

Abstract

Voltage-dependent delayed rectifier K+ (K-V) channels and Ca2+-activated K (K-Ca) channels both play important roles in the regulation of the membrane potential and contractility of vascular smooth muscle cells (SMCs). The expression and function of these K+ channels in cultured vascular SMCs havebeen extensively studied. The long-term in vitro cell culture would changethe properties of K+ channels in SMCs. However, whether the short-term culture could differentially affect the expression and function of K-V and K-Ca channels was not clear. In the present study, both Kv and K-Ca channel currents were identified in freshly dissociated SMCs and in 1-day-cultured SMCs from rat tail arteries. K-Ca currents were inhibited by iberiotoxin or tetraethylammonium (TEA), and amplified by the calcium ionophore A-23187. Kvcurrents were inhibited by 4-aminopyridine or beta -dendrotoxin. By using different pharmacological agents and manipulating the calcium concentrations in the recording solutions, it was revealed that in freshly dissociated SMCs the predominant component of total outward K+ currents is K-Ca current,and K-V current a minor component. In contrast, K-V current was found to be the predominant component of total outward K+ currents in SMCs primarily cultured with 10% fetal bovine serum at 37 degreesC for 24 h. Differential expression of Kv and K-Ca channels in 1-day-cultured SMCs was thus demonstrated under our experimental conditions. Our results are important for interpreting the electrophysiological properties of vascular SMCs under different cell culture conditions and for understanding the relative contributions of Kv and K-Ca channels to different cellular functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 07:27:42