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Titolo:
The constitutive activation of the CEF-4/9E3 chemokine gene depends on C/EBP beta in v-src transformed chicken embryo fibroblasts
Autore:
Gagliardi, M; Maynard, S; Bojovic, B; Bedard, PA;
Indirizzi:
York Univ, Dept Biol, N York, ON M3J 1P3, Canada York Univ N York ON Canada M3J 1P3 Dept Biol, N York, ON M3J 1P3, Canada McMaster Univ, Inst Mol Biol & Biotechnol, Hamilton, ON L8S 4K1, Canada McMaster Univ Hamilton ON Canada L8S 4K1 ol, Hamilton, ON L8S 4K1, Canada
Titolo Testata:
ONCOGENE
fascicolo: 18, volume: 20, anno: 2001,
pagine: 2301 - 2313
SICI:
0950-9232(20010426)20:18<2301:TCAOTC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUS-SARCOMA VIRUS; NECROSIS-FACTOR-ALPHA; NF-KAPPA-B; ENDOTHELIAL GROWTH-FACTOR; EFII ENHANCER SEQUENCE; BINDING-PROTEIN-BETA; LONG TERMINAL REPEAT; KINASE-C SUBSTRATE; TRANSCRIPTION FACTOR; CELLULAR-TRANSFORMATION;
Keywords:
CEF-4/9E3 chemokine; C/EBP beta; pp60(v-src);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Bedard, PA York Univ, Dept Biol, 4700 Keele St, N York, ON M3J 1P3, CanadaYork Univ 4700 Keele St N York ON Canada M3J 1P3 3J 1P3, Canada
Citazione:
M. Gagliardi et al., "The constitutive activation of the CEF-4/9E3 chemokine gene depends on C/EBP beta in v-src transformed chicken embryo fibroblasts", ONCOGENE, 20(18), 2001, pp. 2301-2313

Abstract

The CEF-4/9E3 chemokine gene is expressed constitutively in chicken embryofibroblasts (CEF) transformed by the Rous sarcoma virus (RSV), This aberrant induction is controlled at the transcriptional and posttranscriptional Levels. Transcriptional activation depends on multiple elements of the CEF-4promoter composing a Src-responsive-Unit or SRU, The SRU includes a TPA responsive element, a PRDII/kappaB domain and a CAAT box. In this report, we identify C/EBP beta as a component of the trans-acting factor interacting with the CAAT box of the CEF-4 promoter. In addition, we show that CI EBP beta binds to a second element located in proximity of the TRE, A mutation ofthis distal CAAT box impaired the activation of the CEF-4 promoter by pp60(v-src) indicating that this element is also part of the SRU, Using the RCASBP retroviral vector, we expressed a dominant negative mutant of C/EBP beta (designated Delta 184-C/EBP beta) in RSV-transformed CEF, Delta 184-C/EBPbeta decreased the accumulation of the CEF-4 mRNA and activation of the CEF-4 promoter by pp60(v-src). The induction of the Cox-2 gene (CEF-147) was also reduced by Delta 184-C/EBP beta. The effect of the dominant negative mutant was observed within 1 h of the activation of a thermolabile pp60(v-src) suggesting that C/EBP beta is an early target of v-src transformation. The dominant negative mutant did not inhibit the transformation of CEF by RSV and in fact accentuated the transformed cell phenotype, Therefore, the activation of C/EBP beta is important for the expression of v-src regulated genes but is not required for the in vitro transformation of CEF by pp60(v-src).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:36:14