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Titolo:
Pharmacology, distribution and development of muscarinic acetylcholine receptor subtypes in the optic tectum of Rana pipiens
Autore:
Butt, CM; Pauly, JR; Wilkins, LH; Dwoskin, LP; Debski, EA;
Indirizzi:
Univ Kentucky, Sch Biol Sci, Lexington, KY 40506 USA Univ Kentucky Lexington KY USA 40506 ch Biol Sci, Lexington, KY 40506 USA Univ Kentucky, Coll Pharm, Div Pharmaceut Sci, Lexington, KY 40506 USA Univ Kentucky Lexington KY USA 40506 rmaceut Sci, Lexington, KY 40506 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 1, volume: 104, anno: 2001,
pagine: 161 - 179
SICI:
0306-4522(2001)104:1<161:PDADOM>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHOLINE-ACETYLTRANSFERASE IMMUNOHISTOCHEMISTRY; KITTEN VISUAL-CORTEX; RAT-BRAIN; NUCLEUS-ISTHMI; NICOTINIC ACETYLCHOLINE; BINOCULAR MAPS; AUTORADIOGRAPHIC LOCALIZATION; XENOPUS-LAEVIS; BINDING-SITES; LEOPARD FROG;
Keywords:
cholinergic; visual plasticity; autoradiography; amphibia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
87
Recensione:
Indirizzi per estratti:
Indirizzo: Debski, EA Univ Kentucky, Sch Biol Sci, 101 TH Morgan Bldg, Lexington, KY 40506 USA Univ Kentucky 101 TH Morgan Bldg Lexington KY USA 40506 506 USA
Citazione:
C.M. Butt et al., "Pharmacology, distribution and development of muscarinic acetylcholine receptor subtypes in the optic tectum of Rana pipiens", NEUROSCIENC, 104(1), 2001, pp. 161-179

Abstract

Visually evoked behaviors mediated by the Frog optic tectum require cholinergic activity, but the receptor subtypes through which acetylcholine acts are not yet identified. Using quantitative autoradiography and scintillation spectrometry. we examined the binding of [H-3]pirenzepine and [H-3]AF-DX 384 in the laminated optic tectum of the frog. In mammalian systems, these substances bind excitatory (m1 and m3 subtypes) and inhibitory (m2 and mi subtypes) muscarinic acetylcholine receptors. respectively. Pharmacological analyses, including the use of specific muscarinic toxins, confirmed the subtype selectivity of the radioligands in the frog brain. Binding sires for [H-3]pirenzepine were distinct from those for [H-3]AF-DX 384. In the adult tectum, [H-3]pirenzepine demonstrated specific binding in tectal layers 5-9. [IH]Pirenzepine binding was also present in tadpoles as young as stage V,but all sampled stages of tadpole rectum had significantly less binding when compared to adults. Lesioning of the optic nerve had no effect on [H-3]pirenzepine binding. Specific [H-3]AF-DX 384 binding was found in all layersof the adult tectum. All sampled tadpole stages exhibited binding sites for [H-3]AF-DX 384. but the densities of these sites were also significantly higher in adults than they were in developing stages. Short-term lesions ofthe optic nerve reduced [H-3]AF-DF; 384 binding in all rectal layers of the deafferented lobe when compared to the afferented one. Long-term lesions decreased [H-3]AF-DX 384 sites in both lobes. These results indicate that multiple muscarinic acetylcholine receptor binding sites reside in the frog optic tectum at all stages of development, and their pharmacology resembles that of mammalian m1/m3. m2 and ml subtypes. Our data indicate that few, if any, of these receptors are likely to be located on retinal ganglion cell terminals. Furthermore, the expression of inhibitory muscarinic subtypes seems to be regulated by different mechanisms than that fur excitatory subtypes. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 16:06:06