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Titolo:
Sequential SNARE assembly underlies priming and triggering of exocytosis
Autore:
Chen, YA; Scales, SJ; Scheller, RH;
Indirizzi:
Stanford Univ, Sch Med, Dept Mol & Cellular Physiol, Howard Hughes Med Inst, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ghes Med Inst, Stanford, CA 94305 USA
Titolo Testata:
NEURON
fascicolo: 1, volume: 30, anno: 2001,
pagine: 161 - 170
SICI:
0896-6273(200104)30:1<161:SSAUPA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE-FUSION; SYNAPTIC EXOCYTOSIS; COMPLEX-FORMATION; ALPHA-SNAP; T-SNARE; SYNAPTOTAGMIN; NEUROTOXINS; SPECIFICITY; RESOLUTION; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Scheller, RH Genentech Inc, 1 DNA Way, S San Francisco, CA 94080 USA Genentech Inc 1 DNA Way S San Francisco CA USA 94080 4080 USA
Citazione:
Y.A. Chen et al., "Sequential SNARE assembly underlies priming and triggering of exocytosis", NEURON, 30(1), 2001, pp. 161-170

Abstract

Changes in SNARE conformations during MgATP-dependent priming of cracked PC12 cells were probed by their altered accessibility to various inhibitors. Dominant negative soluble syntaxin and, to a much lesser extent, VAMP coildomains inhibited exocytosis more efficiently after priming. Neurotoxins and an anti-SNAP25 antibody inhibited exocytosis less effectively after priming. We propose that SNAREs partially and reversibly assemble during priming, and that the syntaxin H3 domain is prevented from fully joining the complex until the arrival of the Ca2+ trigger. Furthermore, we find that mutation of hydrophobic residues of the SNAP25 C-terminal coil that contribute toSNARE core interactions affects the maximal rate of exocytosis, while mutation of charged residues on the surface of the complex affects the apparentaffinity of the coil domain for the partially assembled complex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 04:18:05