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Titolo:
A novel technique that measures peptide secretion on a millisecond timescale reveals rapid changes in release
Autore:
Whim, MD; Moss, GWJ;
Indirizzi:
Univ Coll London, Dept Pharmacol, London WC1E 6BT, England Univ Coll London London England WC1E 6BT macol, London WC1E 6BT, England
Titolo Testata:
NEURON
fascicolo: 1, volume: 30, anno: 2001,
pagine: 37 - 50
SICI:
0896-6273(200104)30:1<37:ANTTMP>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE PITUITARY-CELLS; NEUROPEPTIDE-Y; SODIUM-CHANNEL; ATT-20 CELLS; NERVE-TERMINALS; EXPRESSION; EXOCYTOSIS; FMRFAMIDE; VESICLES; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Whim, MD Univ Coll London, Dept Pharmacol, Gower St, London WC1E 6BT, England Univ Coll London Gower St London England WC1E 6BT E 6BT, England
Citazione:
M.D. Whim e G.W.J. Moss, "A novel technique that measures peptide secretion on a millisecond timescale reveals rapid changes in release", NEURON, 30(1), 2001, pp. 37-50

Abstract

Neuropeptides are ubiquitous transmitters that have been implicated in a wide variety of physiological and pathological conditions, and it is important to understand the processes that control their secretion. We have developed a technique that measures neuropeptide secretion with high temporal resolution. This method involves placing an electrophysiological "tag" in a neuropeptide prohormone. The tagged prohormone is subsequently expressed together with an ionotropic receptor that binds the tag. Because the neuropeptide of interest and the tag enter the same population of dense core granules, neuropeptide secretion gives rise to fast, synaptic-like currents. Using this method, we show that peptide secretion can be modulated on a millisecond time scale. This technique could be readily adapted to measure the secretion of any neuropeptide.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:13:40