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Titolo:
Characterization of cis-acting sequences involved in canine adenovirus packaging
Autore:
Soudais, C; Boutin, S; Kremer, EJ;
Indirizzi:
Genethon III, CNRS URA 1923, F-91002 Evry, France Genethon III Evry France F-91002 II, CNRS URA 1923, F-91002 Evry, France
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 631 - 640
SICI:
1525-0016(200104)3:4<631:COCSII>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC RECOMBINATION; RETINOIC ACID RECEPTORS; GENE-THERAPY; IN-VIVO; VIRAL-ANTIGENS; HIGH-CAPACITY; 55-KILODALTON PROTEINS; L1 52-KILODALTON; IMMUNE-RESPONSES; THYROID-HORMONE;
Keywords:
adenovirus; canine; packaging domain; vectors; gene therapy; Cre recombinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Kremer, EJ Genethon III, CNRS URA 1923, 1bis,Rue Int, F-91002 Evry, FranceGenethon III 1bis,Rue Int Evry France F-91002 002 Evry, France
Citazione:
C. Soudais et al., "Characterization of cis-acting sequences involved in canine adenovirus packaging", MOL THER, 3(4), 2001, pp. 631-640

Abstract

The cis-acting packaging domain in adenovirus serotype 5 (Ad5) is a seriesof redundant, albeit not functionally equivalent, "A-repeats" made up of the consensus sequence 5'-TTTCN(8)CG-3'. A-repeats may bind trans-acting factors that direct packaging of the adenovirus genome into the preformed capsid. To try to understand this basic mechanism, we examined the packaging domain from a nonhuman adenovirus. We delimited the canine adenovirus type 2 (CAV-2) packaging domain to within 156 bp via a conditional mutation based on the Cre/IoxP excision. Using an insertion, deletion, and substitution strategy, we generated packaging-defective CAV-2 vectors. Our results demonstrate that, like Ad5, CAV-2 cis-acting packaging sequences are located near the left inverted terminal repeat and are redundant, but not functionally equivalent. However, the bipartite motif found in Ad5 is present only once in CAV-2 and deletion of it caused only a minor variation in the packaging efficiency. We have identified at least four functional cis-acting packagingsequences in CAV-2. The CAV-2 vectors that we generated were not replication-defective in an E1-transcomplementing cell line and as heat stable as the parental vectors that did not contain mutations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:12:08