Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors
Autore:
Hughes, C; Galea-Lauri, J; Farzaneh, F; Darling, D;
Indirizzi:
Guys Kings & St Thomas Sch Med, Rayne Inst, Dept Mol Med, London SE5 9NU, England Guys Kings & St Thomas Sch Med London England SE5 9NU n SE5 9NU, England
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 623 - 630
SICI:
1525-0016(200104)3:4<623:SPPPAC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PACKAGING CELL-LINE; GENE-TRANSFER; HIGH-TITER; STAPHYLOCOCCUS-AUREUS; NUCLEOTIDE-SEQUENCE; ENVELOPE PROTEIN; HUMAN SERUM; IN-VITRO; VIRUS; BINDING;
Keywords:
gene therapy; retrovirus; concentration; paramagnetic; targeting; streptavidin; biotin; lectin; fibronectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Farzaneh, F Guys Kings & St Thomas Sch Med, Rayne Inst, Dept Mol Med, 123 Coldharbour Lane, London SE5 9NU, England Guys Kings & St Thomas Sch Med 123 Coldharbour Lane London England SE5 9NU
Citazione:
C. Hughes et al., "Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors", MOL THER, 3(4), 2001, pp. 623-630

Abstract

Three strategies have been designed to concentrate infectious retroviral vectors from the supernatants of human- (HT1080) and murine- (NIH 3T3) basedpackaging cells. Streptavidin-conjugated paramagnetic particles in conjunction with (i) antibodies directed against murine fibronectin, (ii) biotinylated lectins, or (iii) biotin-modified packaging cell-surface proteins allow affinity-mediated magnetic concentration of retroviral vectors. Retroviral titers (assayed by colony formation of human myeloid K562 cells) are increased by 1-4 x 10(3)-fold after volume reductions of only 125-fold. Using these procedures, preparations of 5 x 10(8) cfu/ml are routinely made from relatively low-titer (2-5 x 10(5) du/ml) starting material. High-titer (paramagnetic) retroviral vector preparations can be used for magnetic field-dependent retroviral infection in vitro. Magnetic field-dependent localizationsuch as this may enable the in vivo administration of formulations that concentrate retroviral infection to the required target tissues and organs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/02/20 alle ore 22:56:03