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Titolo:
A Cre-expressing cell line and an E1/E2a double-deleted virus for preparation of helper-dependent adenovirus vector
Autore:
Zhou, HS; Zhao, TJ; Pastore, L; Nageh, M; Zheng, W; Rao, XM; Beaudet, AL;
Indirizzi:
Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Gene Therapy, Houston, TX 77030 USA Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA Baylor CollMed Houston TX USA 77030 & Human Genet, Houston, TX 77030 USA Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 , Dept Pediat, Houston, TX 77030 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 613 - 622
SICI:
1525-0016(200104)3:4<613:ACCLAA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPLICATION-COMPETENT ADENOVIRUS; GENE-TRANSFER; IN-VIVO; TRANSGENE EXPRESSION; MEDIATED TRANSFER; MUSCLE-CELLS; SYSTEM; RECOMBINANT; DNA; TYPE-5;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Zhou, HS Baylor Coll Med, Ctr Cell & Gene Therapy, 1 Baylor Plaza,Room N1010, Houston, TX 77030 USA Baylor Coll Med 1 Baylor Plaza,Room N1010 HoustonTX USA 77030 SA
Citazione:
H.S. Zhou et al., "A Cre-expressing cell line and an E1/E2a double-deleted virus for preparation of helper-dependent adenovirus vector", MOL THER, 3(4), 2001, pp. 613-622

Abstract

Adenoviral vectors are attractive for the delivery of transgenes into mammalian cells because of their efficient transduction, high titer, and stability. The major concerns with using El-deleted adenoviral vectors in gene therapy are the pathogenic potential of the virus backbone and the leaky viral protein synthesis that leads to host immune responses and a short duration of transgene expression. Helper-dependent (HD) adenoviral vectors that are devoid of all viral protein-coding sequences have significantly increasedthe safety and reduced the immunogenicity of these vectors. Currently available HD vectors depend on an El-deleted adenovirus as a helper to provide viral proteins in trans. As a consequence, contamination with helper virus cannot be avoided in the HD vector preparation though it can be decreased to 0.01% using a Cre/IoxP mechanism. Since the presence of El-deleted helpervirus may have substantial unwanted effects, we have developed a new Cre-expressing cell line based on an E1- and E2a-complementing cell. This new cell line can efficiently cleave the packaging region in the helper virus genome. We have also developed an El and E2a double-deleted helper virus. By using the CreE cell with the helper virus deleted in both the El and the E2agenes it may be possible to further improve the safety of the vectors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 02:51:20