Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Lipopolysaccharide potentiates the effect of hepatocyte growth factor uponreplication in lung, thyroid, spleen, and colon in rats in vivo
Autore:
Gao, CH; Kennedy, S; Ponder, KP;
Indirizzi:
Washington Univ, Sch Med, Dept Internal Med, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 nternal Med, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Mol Biophys, St Louis, MO 63110 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 462 - 475
SICI:
1525-0016(200104)3:4<462:LPTEOH>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR SCATTER FACTOR; EPITHELIAL-CELL PROLIFERATION; FACTOR RECEPTOR EXPRESSION; ACUTE-RENAL-FAILURE; C-MET; SKELETAL-MUSCLE; RETROVIRAL VECTOR; ADENOVIRUS VECTORS; PANCREATIC-CANCER; SATELLITE CELLS;
Keywords:
bromodeoxyuridine; labeling index; gene therapy; retroviral vector;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Ponder, KP Washington Univ, Sch Med, Dept Internal Med, 660 S Euclid Ave,Box 8125, StLouis, MO 63110 USA Washington Univ 660 S Euclid Ave,Box 8125 StLouis MO USA 63110
Citazione:
C.H. Gao et al., "Lipopolysaccharide potentiates the effect of hepatocyte growth factor uponreplication in lung, thyroid, spleen, and colon in rats in vivo", MOL THER, 3(4), 2001, pp. 462-475

Abstract

Induction of replication may potentiate in vivo gene therapy, as some viral vectors only transduce dividing cells. Hepatocyte growth factor (HGF) increases the percentage of replicating hepatocytes to 18-fold that in normal rats, and lipopolysaccharide (LPS) modestly potentiates this effect. In this study, the effect of iv HGF upon replication in other organs was determined. HGF at 10 mg/kg resulted in replication that was less than or equal to3-fold that of normal rats in alveolar and proximal renal tubular cells. HGFalone had no effect upon replication of epithelial cells from the bronchi,thyroid, pancreas, or colon or upon cells from the muscle, pancreatic islets, spleen, blood vessels, or thymus. HGF and LPS at 5 mg/kg resulted in replication that was 9-fold that of normal rats in alveolar cells, 25-fold inbronchial epithelial cells, 4-fold in thyroid epithelial cells, 1.5-fold in the red pulp of the spleen, and 2-fold in colonic epithelial cells. The synergistic effect may be due to the fact that LPS upregulated the HCF receptor c-met in thyroid, spleen, and colon. We conclude that iv administrationof HGF alone is relatively specific for inducing hepatocyte replication and would allow selective gene transfer into the liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 15:03:43