Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Enhanced transgene expression in cord blood CD34(+)-derived hematopoietic cells, including developing T cells and NOD/SCID mouse repopulating cells, following transduction with modified TRIP lentiviral vectors
Autore:
Sirven, A; Ravet, E; Charneau, P; Zennou, V; Coulombel, L; Guetard, D; Pflumio, F; Dubart-Kupperschmitt, A;
Indirizzi:
Inst Gustave Roussy, INSERM, U362, F-94805 Villejuif, France Inst Gustave Roussy Villejuif France F-94805 , F-94805 Villejuif, France Inst Pasteur, Unite Oncol Virale, F-75724 Paris 15, France Inst Pasteur Paris France 15 nite Oncol Virale, F-75724 Paris 15, France
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 438 - 448
SICI:
1525-0016(200104)3:4<438:ETEICB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; HUMAN CD34(+) CELLS; LONG-TERM CULTURES; STEM-CELLS; IN-VIVO; PROGENITOR CELLS; STABLE TRANSDUCTION; BONE-MARROW; RETROVIRAL VECTORS; NUCLEAR IMPORT;
Keywords:
lentiviral vectors; central DNA flap; gene transfer; human hematopoietic stem cells; transgene expression; EF1 alpha promoter; T cell differentiation; NOD/SCID mouse repopulating cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Dubart-Kupperschmitt, A INSERM, U474, 123 Blvd Port Royal, F-75014 Paris, France INSERM 123 Blvd Port Royal Paris France F-75014 e
Citazione:
A. Sirven et al., "Enhanced transgene expression in cord blood CD34(+)-derived hematopoietic cells, including developing T cells and NOD/SCID mouse repopulating cells, following transduction with modified TRIP lentiviral vectors", MOL THER, 3(4), 2001, pp. 438-448

Abstract

The recent development of lentivirus-derived vectors is an important breakthrough in gene transfer technology because these vectors allow transduction of nondividing cells such as hematopoietic stem cells (HSC), due to an active nuclear import of reverse-transcribed vector DNA. We recently demonstrated that addition of the central DNA flap of HIV-1 to an HIV-derived lentiviral vector strikingly increases transduction of CD34(+) cells. We now describe improvements of the transduction protocol designed to preserve HSC properties and two modifications of the previously described TRIP-CMV vector. First, deletion of the enhancer/promotor of the 3 ' LTR in the TRIP-CMV vector resulted in a safer vector (TRIP Delta U3-CMV) with conserved transduction efficiency and increased EGFP transgene expression. Second, the original internal CMV promoter was replaced with the promoter for the ubiquitously expressed elongation factor 1 alpha (EF1 alpha). This promoter substitution resulted in a significantly more homogeneous expression of the EGFP transgene in all hematopoietic cell types, including CD34(+)-derived T lymphocytes, in which the CMV promoter was inactive, and NOD/SCID mouse repopulating cells. We thus present here an HIV-derived lentiviral vector, TRIP Delta U3-EF1 alpha, which can very efficiently transduce human cord blood HSC andresults in high long-term transgene expression in CD34(+)-derived T, B, NK, and myeloid hematopoietic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:18:10