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Titolo:
Porcine oocyte activation: Differing roles of calcium and pH
Autore:
Ruddock, NT; Machaty, Z; Cabot, RA; Prather, RS;
Indirizzi:
Univ Missouri, Dept Anim Sci, Anim Sci Res Ctr 162, Columbia, MO 65211 USAUniv Missouri Columbia MO USA 65211 i Res Ctr 162, Columbia, MO 65211 USA
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 59, anno: 2001,
pagine: 227 - 234
SICI:
1040-452X(200106)59:2<227:POADRO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR PH; EGG ACTIVATION; PIG OOCYTES; IN-VITRO; PARTHENOGENETIC ACTIVATION; FLUOROCHROME HOECHST-33342; MOUSE OOCYTES; FERTILIZATION; SPERM; EMBRYO;
Keywords:
pH; calcium; oocyte; pig; activation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Prather, RS Univ Missouri, Dept Anim Sci, Anim Sci Res Ctr 162, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 2, Columbia, MO 65211 USA
Citazione:
N.T. Ruddock et al., "Porcine oocyte activation: Differing roles of calcium and pH", MOL REPROD, 59(2), 2001, pp. 227-234

Abstract

Intracellular pH has recently been shown to increase during parthenogenetic activation of the porcine oocyte. In the following set of experiments, intracellular pH was monitored during activation and pronuclear development was assessed following activation treatments with calcium, in the absence ofcalcium, and in oocytes loaded with the calcium chelator BAPTA-AM in calcium-free medium. Intracellular pH increase was not different among groups when treating with 7% ethanol or 50 muM calcium ionophore, or during treatment with thimerosal for 12 or 25 min. Activation with thimerosal (200 muM, 12min) followed by 8 mM dithiothreitol (DTT, 30 min) resulted in a decreasedpronuclear development in calcium-free medium with or without BAPTA-AM loaded oocytes as compared to controls. Activation with 50 muM calcium ionophore resulted in pronuclear development that was different between the calcium-free and BAPTA-AM loaded oocytes in calcium-free medium. Similar incidences of pronuclear formation were observed in all ethanol treatment groups. It was concluded that external calcium as well as large changes in intracellular free calcium are not necessary for the increase in intracellular pH, but normal intracellular calcium signaling is critical for normal levels of pronuclear development. Finally, oocytes were measured for intracellular pHchanges for 30 min following subzonal sperm injection. Intracellular pH did not increase, although pronuclear formation was observed 6 hr post SUZI. This suggested that major differences were still present between sperm-induced and parthenogenetic activation of the porcine oocyte. Mol. Reprod. Dev.59:227-234, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:11:10