Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Essential role of the nonreducing terminal alpha-mannosyl residues of the N-linked carbohydrate chain of bovine zona pellucida glycoproteins in sperm-egg binding
Autore:
Amari, S; Yonezawa, N; Mitsui, S; Katsumata, T; Hamano, S; Kuwayama, M; Hashimoto, Y; Suzuki, A; Takeda, Y; Nakano, M;
Indirizzi:
Chiba Univ, Fac Sci, Dept Chem, Inage Ku, Chiba 2638522, Japan Chiba UnivChiba Japan 2638522 Dept Chem, Inage Ku, Chiba 2638522, Japan Chiba Univ, Grad Sch Sci & Technol, Chiba 260, Japan Chiba Univ Chiba Japan 260 niv, Grad Sch Sci & Technol, Chiba 260, Japan Livestock Improvement Assoc, Anim Biotechnol Ctr, Tokyo, Japan Livestock Improvement Assoc Tokyo Japan im Biotechnol Ctr, Tokyo, Japan
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 59, anno: 2001,
pagine: 221 - 226
SICI:
1040-452X(200106)59:2<221:EROTNT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR ACTIVITY; FERTILIZED-EGGS; INVITRO; ZP3; OLIGOSACCHARIDES; CAPACITATION; SPERMATOZOA; INVOLVEMENT; FAMILIES; OVARIAN;
Keywords:
glycoprotein; in vitro fertilization; sperm-egg binding; sperm ligand; zona pellucida;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Nakano, M Chiba Univ, Fac Sci, Dept Chem, Inage Ku, 1-33 Yayoi Cho, Chiba 2638522, Japan Chiba Univ 1-33 Yayoi Cho Chiba Japan 2638522 ba 2638522, Japan
Citazione:
S. Amari et al., "Essential role of the nonreducing terminal alpha-mannosyl residues of the N-linked carbohydrate chain of bovine zona pellucida glycoproteins in sperm-egg binding", MOL REPROD, 59(2), 2001, pp. 221-226

Abstract

It has been proposed that mammalian sperm bind species-specifically to carbohydrate chains of zona pellucida glycoproteins at fertilization. Althoughthe sperm ligand carbohydrate chains have been characterized in mice and pigs, the existence of the ligands of other mammals remains unclear. In order to explore the bovine sperm ligand, two in vitro competition assay methods were applied. As a result, a high-mannose-type carbohydrate chain, Man alpha1-6(Man alpha1-3)Man alpha1-6(Man alpha1-3)Man beta1-4GlcNAc beta1- 4GlcNAc, which is the major neutral chain in bovine egg zona glycoproteins, wasshown to possess bovine sperm ligand activity. When nonreducing terminal alpha -mannosyl residues were eliminated from the zona glycoproteins by alpha -mannosidase digestion, the ligand activity was reduced, indicating that the alpha -mannosyl residues play an essential role in bovine sperm-egg binding. The number of sperm binding to eggs was reduced to about one-half after fertilization. The ligand active high-mannose-type chain may be buried after fertilization, since its amount remains unchanged. Pretreatment of bovine sperm with the sperm ligand-carbohydrate chain significantly inhibited penetration of the sperm into oocyte and the male pronucleus formation. Thus, a correlation between the sperm ligand activity and in vitro fertilization rate was observed. Mol. Reprod. Dev. 59:221-226, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:17:17