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Titolo:
Transcriptional regulation of vascular endothelial growth factor expression in epithelial and stromal cells during mouse mammary gland development
Autore:
Hovey, RC; Goldhar, AS; Baffi, J; Vonderhaar, BK;
Indirizzi:
Natl Canc Inst, Tumor Immunol & Biol Lab, NIH, Bethesda, MD 20892 USA NatlCanc Inst Bethesda MD USA 20892 iol Lab, NIH, Bethesda, MD 20892 USA NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA NEI Bethesda MD USA 20892NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 5, volume: 15, anno: 2001,
pagine: 819 - 831
SICI:
0888-8809(200105)15:5<819:TROVEG>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILITY FACTOR EXPRESSION; TUMOR ANGIOGENESIS; FACTOR FAMILY; FAT-CELLS; VEGF; MICE; RECEPTOR; ESTROGEN; GENE; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Vonderhaar, BK Natl Canc Inst, Tumor Immunol & Biol Lab, NIH, Bldg 10,Room5B47,10 Ct Dr,Bethesda, MD 20892 USA Natl Canc Inst Bldg 10,Room 5B47,10 Ct Dr Bethesda MD USA 20892
Citazione:
R.C. Hovey et al., "Transcriptional regulation of vascular endothelial growth factor expression in epithelial and stromal cells during mouse mammary gland development", MOL ENDOCR, 15(5), 2001, pp. 819-831

Abstract

Accompanying changes in the development and function of the mammary gland is the establishment of a vascular network of critical importance for lactogenesis and tumorigenesis. A potent angiogenic and permeability factor thatregulates vascular development in association with epithelial-stromal interactions is vascular endothelial growth factor (VEGF), Analysis of VEGF transcription by RT-PCR revealed mRNA for all three VEGF isoforms (VEGF(120), (164), (188)) within the mammary gland of nulliparous females. During pregnancy the level of VEGF(188) declined and became undetectable during lactation in association with the increased abundance of VEGF(120) and VEGF(164) mRNAs, All three isoforms were expressed at consistent levels within the cleared mammary fat pad throughout development. Furthermore, the presence of VEGF(188) mRNA in omental adipose tissue at various stages established that VEGF(188) is expressed specifically in adipose tissue within the mammary gland, Using 3T3-L1 preadipocytes it was demonstrated that VEGF(188) mRNA transcription occurs as a late event during lipogenesis distinct from earlier induction of VEGF(120) and VEGF(164) mRNA during differentiation. In contrast, HC11 mammary epithelial cells only expressed mRNA for VEGF(120) and VEGF(164). Localization of VEGF mRNA and protein revealed that VEGF is expressed in stromal cells of the mammary gland in nulliparous females and then undergoes a transition to epithelial expression during lactation. By contrast, mRNA for the VEGF receptors, Flk-1 and Flt-1, localized to stromal cells within the mammary fat pad during virgin and gestational development and was expressed in the interstitial tissue basal to epithelial cells during lactation. Taken together, these results support the conclusion that VEGF is differentially transcribed by specific cell types within the mammary gland, and that under hormonal regulation it functions in an autocrine/paracrine manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:20:11