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Titolo:
Heterochromatin formation in mammalian cells: Interaction between histonesand HP1 proteins
Autore:
Nielsen, AL; Oulad-Abdelghani, M; Ortiz, JA; Remboutsika, E; Chambon, P; Losson, R;
Indirizzi:
Coll France, ULP, CNRS,INSERM, Inst Genet & Biol Mol & Cellulaire, F-67404Illkirch, France Coll France Illkirch France F-67404 Cellulaire, F-67404Illkirch, France Aarhus Univ, Inst Human Genet, DK-8000 Aarhus C, Denmark Aarhus Univ Aarhus Denmark C Inst Human Genet, DK-8000 Aarhus C, Denmark Aarhus Univ, Dept Biol Mol & Struct, DK-8000 Aarhus C, Denmark Aarhus Univ Aarhus Denmark C iol Mol & Struct, DK-8000 Aarhus C, Denmark Natl Inst Med Res, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA ed Res, London NW7 1AA, England
Titolo Testata:
MOLECULAR CELL
fascicolo: 4, volume: 7, anno: 2001,
pagine: 729 - 739
SICI:
1097-2765(200104)7:4<729:HFIMCI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITION-EFFECT VARIEGATION; CHROMO DOMAIN; DROSOPHILA-MELANOGASTER; IN-VITRO; COMPLEX; ASSOCIATION; MOUSE; NUCLEOSOME; BINDING; RECOGNITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Chambon, P Coll France, ULP, CNRS,INSERM, Inst Genet & Biol Mol & Cellulaire, BP163, F-67404 Illkirch, France Coll France BP163 Illkirch France F-67404 404 Illkirch, France
Citazione:
A.L. Nielsen et al., "Heterochromatin formation in mammalian cells: Interaction between histonesand HP1 proteins", MOL CELL, 7(4), 2001, pp. 729-739

Abstract

Members of the heterochromatin protein 1 (HP1) family are silencing nonhistone proteins. Here, we show that in P-19 embryonal carcinoma (EC) nuclei, HP1 alpha, beta, and gamma form homo- and heteromers associated with nucleosomal core histones. In vitro, all three HP1s bind to tailed and tailless nucleosomes and specifically interact with the histone-fold of histone H3. Furthermore, HP1 alpha interacts with the linker histone H1. HP1 alpha bindsto H3 and H1 through its chromodomain (CD) and hinge region, respectively. Interestingly, the Polycomb (Pc1/M33) CD also interacts with H3, and HP1 alpha and Pc1/M33 binding to H3 is severely impaired by CD mutations known to abrogate HP1 and Polycomb silencing in Drosophila. These results define anovel function for the conserved CD and suggest that HP1 self-association and histone binding may play a crucial role in HP1-mediated heterochromatinassembly.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:44:49