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Titolo:
Cascade of caspase activation in potassium-deprived cerebellar granule neurons: Targets for treatment with peptide and protein inhibitors of apoptosis
Autore:
Gerhardt, E; Kugler, S; Leist, M; Beier, C; Berliocchi, L; Volbracht, C; Weller, M; Bahr, M; Nicotera, P; Schulz, JB;
Indirizzi:
Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany Univ Tubingen Tubingen Germany l, Lab Neurodegenerat, Tubingen, Germany Univ Tubingen, Dept Neurol, Lab Neurooncol, Tubingen, Germany Univ Tubingen Tubingen Germany eurol, Lab Neurooncol, Tubingen, Germany Univ Tubingen, Sch Med, Tubingen, Germany Univ Tubingen Tubingen Germany niv Tubingen, Sch Med, Tubingen, Germany Univ Konstanz, Dept Toxicol, D-7750 Constance, Germany Univ Konstanz Constance Germany D-7750 oxicol, D-7750 Constance, Germany
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 4, volume: 17, anno: 2001,
pagine: 717 - 731
SICI:
1044-7431(200104)17:4<717:COCAIP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; SIGNALING COMPLEX DISC; BACULOVIRUS P35; CYTOCHROME-C; COMBINATORIAL APPROACH; RAT HIPPOCAMPUS; NERVOUS-SYSTEM; CD95 LIGAND; FAS LIGAND; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Schulz, JB Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany Univ Tubingen Tubingen Germany odegenerat, Tubingen, Germany
Citazione:
E. Gerhardt et al., "Cascade of caspase activation in potassium-deprived cerebellar granule neurons: Targets for treatment with peptide and protein inhibitors of apoptosis", MOL CELL NE, 17(4), 2001, pp. 717-731

Abstract

Cerebellar granule neurons (CGN) cultured in the presence of serum and depolarizing potassium concentrations undergo apoptosis when switched to serum-free medium containing physiological potassium concentrations. Here we show that processing of the key protease, caspase-3, depends on the activationof caspase-9, but not of caspase-8. Selective peptide inhibitors of caspase-9 block processing of caspase-3 and caspase-8 and inhibit apoptosis, whereas a selective inhibitor of caspase-8 blocks neither processing of caspase-3 nor cell death. The data obtained with peptide inhibitors were confirmedby adenovirally mediated ectopic expression of the cytokine response modifier A (crmA), the baculovirus protein p35, and the X chromosome-linked inhibitor of apoptosis (XIAP). Further, caspase-8-activating death receptors donot mediate apoptosis in CGN and potassium withdrawal-induced apoptosis evolves unaltered in gld or lpr mice, which harbor mutations in the CD95/CD95ligand system. Thus, neuronal apoptosis triggered by potassium deprivationis death receptor-independent but involves the mitochondrial pathway of caspase activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 07:47:40