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Titolo:
In vivo internalization of the somatostatin sst2A receptor in rat brain: Evidence for translocation of cell-surface receptors into the endosomal recycling pathway
Autore:
Csaba, Z; Bernard, V; Helboe, L; Bluet-Pajot, MT; Bloch, B; Epelbaum, J; Dournaud, P;
Indirizzi:
Ctr Paul Broca, IFR Broca St Anne, INSERM, U549, F-75014 Paris, France CtrPaul Broca Paris France F-75014 INSERM, U549, F-75014 Paris, France Univ Bordeaux 2, Lab Histol Embryol, CNRS, UMR 5541, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 R 5541, F-33076 Bordeaux, France Univ Copenhagen, Panum Inst, Dept Med Anat, DK-2200 Copenhagen, Denmark Univ Copenhagen Copenhagen Denmark DK-2200 , DK-2200 Copenhagen, Denmark
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 4, volume: 17, anno: 2001,
pagine: 646 - 661
SICI:
1044-7431(200104)17:4<646:IVIOTS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURONS IN-VIVO; MUSCARINIC ACETYLCHOLINE-RECEPTORS; PROTEIN-COUPLED RECEPTORS; CENTRAL-NERVOUS-SYSTEM; MU-OPIOID RECEPTOR; IMMUNOHISTOCHEMICAL LOCALIZATION; SPINAL-CORD; SUBSTANCE-P; MOLECULAR PHARMACOLOGY; HIPPOCAMPAL-NEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Csaba, Z Ctr Paul Broca, IFR Broca St Anne, INSERM, U549, F-75014 Paris, France Ctr Paul Broca Paris France F-75014 U549, F-75014 Paris, France
Citazione:
Z. Csaba et al., "In vivo internalization of the somatostatin sst2A receptor in rat brain: Evidence for translocation of cell-surface receptors into the endosomal recycling pathway", MOL CELL NE, 17(4), 2001, pp. 646-661

Abstract

To determine whether cellular compartmentalization of somatostatin receptors can be regulated in vivo, we examined the immunocytochemical distribution of the sst2A receptor (sst2AR) after stereotaxical injections of somatostatin analogs into the rat parietal cortex. Whereas CH-275, a sst1R agonist,failed to induce changes in the diffuse sst2AR immunostaining pattern characteristic of control animals, somatodendritic profiles displaying intracytoplasmic immunoreactive granules became apparent short-term after injectionof either somatostatin or the sst2R agonist octreotide. Confocal microscopy revealed that 90% of sst2AR-immunoreactive endosome-like organelles displayed transferrin receptor immunoreactivity. At the electron microscopic level, the percentage of sst2AR immunoparticles dramatically decreased at the plasmalemma of perikarya and dendrites after octreotide injection. Conversely, it significantly increased in endosomes-like organelles. These results demonstrate that sst2ARs undergo, in vivo, rapid and massive internalization into the endocytic recycling compartment in response to acute agonist stimulation and provide important clues toward elucidating somatostatin receptor signaling in the mammalian brain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:25:55