Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The neuron-restrictive silencer element-neuron-restrictive silencer factorsystem regulates basal and endothelin 1-inducible atrial natriuretic peptide gene expression in ventricular myocytes
Autore:
Kuwahara, K; Saito, Y; Ogawa, E; Takahashi, N; Nakagawa, Y; Naruse, Y; Harada, M; Hamanaka, I; Izumi, T; Miyamoto, Y; Kishimoto, I; Kawakami, R; Nakanishi, M; Mori, N; Nakao, K;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Med & Clin Sci, Sakyo Ku, Kyoto 6068397, Japan Kyoto Univ Kyoto Japan 6068397 Clin Sci, Sakyo Ku, Kyoto 6068397, Japan Natl Inst Longev Sci, Dept Mol Genet Res, Aichi 4748522, Japan Natl Inst Longev Sci Aichi Japan 4748522 Genet Res, Aichi 4748522, Japan JST, Sci & Technol Corp Japan, CREST, Kawaguchi, Saitama 3320012, Japan JST Kawaguchi Saitama Japan 3320012 ST, Kawaguchi, Saitama 3320012, Japan
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 6, volume: 21, anno: 2001,
pagine: 2085 - 2097
SICI:
0270-7306(200103)21:6<2085:TNSESF>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM-CHANNEL GENE; TRANSCRIPTION FACTORS; CARDIOCYTE HYPERTROPHY; RECOMBINANT ADENOVIRUS; AUGMENTED EXPRESSION; HISTONE DEACETYLASE; POLYPEPTIDE GENE; CARDIAC MYOCYTES; RECEPTOR GENE; MESSENGER-RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Saito, Y Kyoto Univ, Grad Sch Med, Dept Med & Clin Sci, Sakyo Ku, 54 Shogoin Kawahara Cho, Kyoto 6068397, Japan Kyoto Univ 54 Shogoin Kawahara Cho Kyoto Japan 6068397 97, Japan
Citazione:
K. Kuwahara et al., "The neuron-restrictive silencer element-neuron-restrictive silencer factorsystem regulates basal and endothelin 1-inducible atrial natriuretic peptide gene expression in ventricular myocytes", MOL CELL B, 21(6), 2001, pp. 2085-2097

Abstract

Induction of the atrial natriuretic peptide (ANP) gene is a common featureof ventricular hypertrophy. A number of cis acting enhancer elements for several transcriptional activators have been shown to play central roles in the regulation of ANP gene expression, but much less is known about contributions made by transcriptional repressors. The neuron-restrictive silencer element (NRSE), also known as repressor element 1, mediates repression of neuronal gene expression in nonneuronal cells. We found that NRSE, which is located in the 3 ' untranslated region of the ANP gene, mediated repressionof ANP promoter activity in ventricular myocytes and was also involved in the endothelin 1-induced increase in ANP gene transcription. The repressionwas conferred by a repressor protein, neuron-restrictive silencer factor (NRSF). NRSF associated with the transcriptional corepressor mSin3 and formed a complex with histone deacetylase (HDAC) in ventricular myocytes. Trichostatin A (TSA), a specific HDAC inhibitor, relieved NRSE-mediated repression of ANP promoter activity, and chromatin immunoprecipitation assays revealed the involvement of histone deacetylation in NRSE-mediated repression of ANP gene expression. Furthermore, in myocytes infected with recombinant adenovirus expressing a dominant-negative form of NRSF, the basal level of endogenous ANP gene expression,vas increased and a TSA-induced increase in ANPgene expression was apparently attenuated, compared with those in myocytesinfected with control adenovirus. Our findings show that an NRSE-NRSF system plays a key role in the regulation of ANP gene expression by HDAC in ventricular myocytes and provide a new insight into the role of the NRSE-NRSF system outside the nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 08:38:34