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Titolo:
DNA fingerprinting of serial Candida albicans isolates obtained during itraconazole prophylaxis in patients with AIDS
Autore:
Le Monte, AM; Goldman, M; Smedema, ML; Connolly, PA; McKinsey, DS; Cloud, GA; Kauffman, CA; Wheat, LJ;
Indirizzi:
Indiana Univ, Sch Med, Histoplasmosis Reference Lab, OPW 430, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 PW 430, Indianapolis, IN 46202 USA Histoplasmosis Reference Lab, Indianapolis, IN USA Histoplasmosis Reference Lab Indianapolis IN USA b, Indianapolis, IN USA Med Res Ctr, Kansas City, MO USA Med Res Ctr Kansas City MO USAMed Res Ctr, Kansas City, MO USA Univ Alabama, Birmingham, AL USA Univ Alabama Birmingham AL USAUniv Alabama, Birmingham, AL USA Univ Michigan, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 iv Michigan, Ann Arbor, MI 48109 USA Vet Affairs Med Ctr, Ann Arbor, MI USA Vet Affairs Med Ctr Ann Arbor MI USA Affairs Med Ctr, Ann Arbor, MI USA Richard L Roudebush Vet Affairs Med Ctr, Indianapolis, IN 46202 USA Richard L Roudebush Vet Affairs Med Ctr Indianapolis IN USA 46202 202 USA
Titolo Testata:
MEDICAL MYCOLOGY
fascicolo: 2, volume: 39, anno: 2001,
pagine: 207 - 213
SICI:
1369-3786(200104)39:2<207:DFOSCA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIV-INFECTED PATIENTS; IMMUNODEFICIENCY-VIRUS INFECTION; MOLECULAR TYPING METHODS; IN-VITRO SUSCEPTIBILITY; ORAL CANDIDIASIS; OROPHARYNGEAL CANDIDIASIS; FLUCONAZOLE RESISTANCE; POSITIVE PATIENTS; DOUBLE-BLIND; STRAINS;
Keywords:
AIDS; Candida; fingerprinting; itraconazde;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Le Monte, AM Indiana Univ, Sch Med, Histoplasmosis Reference Lab, OPW 430,1001 W 10th St, Indianapolis, IN 46202 USA Indiana Univ 1001 W 10th St Indianapolis IN USA 46202 202 USA
Citazione:
A.M. Le Monte et al., "DNA fingerprinting of serial Candida albicans isolates obtained during itraconazole prophylaxis in patients with AIDS", MED MYCOL, 39(2), 2001, pp. 207-213

Abstract

During a randomized double-blind placebo-controlled study testing the efficacy of itraconazole for prophylaxis of systemic and mucosal fungal infections in patients with acquired immune deficiency syndrome, 298 patients wereenrolled with 295 evaluable. Of those, 46 patients were considered prophylaxis failures because of recurrent oral or esophageal candidiasis. Oropharyngeal fungal cultures were taken at the time of suspected thrush or Cannidaesophagitis, but not at baseline. All of the Candida spp, isolates were cultured on CHROMagar Candida medium then identified using API 20 AUX strips. Antifungal susceptibility testing was performed following the National Committee for Clinical Laboratory Standards M-27A guidelines. Sequential isolates were genotyped using randomly amplified poly morphic DNA, Polymerase chain reaction fingerprints were generated using two repetitive sequence primers, (GGA)(7) and (GACA)(4). The study group consisted of 23 patients, ninefrom the itraconazole arm and 14 from the placebo arm, who were prophylaxis failures and had more than two C, albicans isolates. Five of 23 had isolates showing a greater than or equal to4-fold reduction in susceptibility: four of these patients were in the itraconazole prophylaxis arm and one was in the placebo arm. Three of the five had yeast isolations showing changes in banding patterns over time. Such changes may indicate genetic changes inthe same strain that could be linked to acquired resistance to itraconazole, or acquisition of a new strain, or emergence of a previously minor component of the original population.

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Documento generato il 13/07/20 alle ore 05:51:29