Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Real-time measurements of cellular oxygen consumption, pH, and energy metabolism using nuclear magnetic resonance spectroscopy
Autore:
Pilatus, U; Aboagye, E; Artemov, D; Mori, N; Ackerstaff, E; Bhujwalla, ZM;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Radiol, Div MR Res,MR Oncol Sect, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 l Sect, Baltimore, MD 21205 USA
Titolo Testata:
MAGNETIC RESONANCE IN MEDICINE
fascicolo: 5, volume: 45, anno: 2001,
pagine: 749 - 755
SICI:
0740-3194(200105)45:5<749:RMOCOC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR-PH; TUMOR METABOLISM; CELLS; LONIDAMINE; TENSION; GROWTH;
Keywords:
cancer cells; on-line oxygen measurements; NMR spectroscopy; oxygen consumption; cell metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Bhujwalla, ZM Johns Hopkins Univ, Sch Med, Dept Radiol, Div MR Res,MR Oncol Sect, Rm 208C,Traylor Bldg,720 Ruthland Ave, Baltimore, MD 21205 USA Johns Hopkins Univ Rm 208C,Traylor Bldg,720 Ruthland Ave Baltimore MD USA 21205
Citazione:
U. Pilatus et al., "Real-time measurements of cellular oxygen consumption, pH, and energy metabolism using nuclear magnetic resonance spectroscopy", MAGN RES M, 45(5), 2001, pp. 749-755

Abstract

Changes in molecular expression or apoptotic behavior, induced by malignant transformation or anticancer treatment, are frequently reflected in cellular metabolism and oxygen consumption. A technique to monitor oxygen consumption, cell physiology, and metabolism noninvasively would provide a betterunderstanding of interactions between molecular changes and metabolism in malignant transformation and following cancer treatment. Such a system was developed in this study by adapting multinuclear MRI and spectroscopic techniques to an isolated cell perfusion system. The system was evaluated by studying the effects of two agents, carbonyl cyanide m-chlorophenylhydrazone (CCCP) which is an uncoupler of oxidative phosphorylation, and antimycin, an inhibitor of oxidative phosphorylation, on the oxygen consumption and metabolism of MCF-7 and MatLyLu cancer cell lines. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:29:50