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Titolo:
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes (Reprinted from EMBO Journal, vol 4, pg 1609-1614, 1985)
Autore:
Miller, J; McLachlan, AD; Klug, A;
Titolo Testata:
JOURNAL OF TRACE ELEMENTS IN EXPERIMENTAL MEDICINE
fascicolo: 2, volume: 14, anno: 2001,
pagine: 157 - 169
SICI:
0896-548X(2001)14:2<157:RZDITP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
5S RNA GENE; DIRECTS SPECIFIC INITIATION; CONTROL REGION; COMPLEXES; REFINEMENT; SEQUENCE; BORDER; MODEL;
Keywords:
Xenopus laevis; transcription factor IIIA; zinc binding domains; 7S particle;
Tipo documento:
Reprint
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J. Miller et al., "Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes (Reprinted from EMBO Journal, vol 4, pg 1609-1614, 1985)", J TR EL EXP, 14(2), 2001, pp. 157-169

Abstract

The 7S particle of Xenopus laevis oocytes contains 5S RNA and a 40-K protein which is required for 5S RNA transcription in vitro. Proteolytic digestion of the protein in the particle yields periodic intermediates spaced at 3-K intervals and a limit digest containing 3-K fragments. The native particle is shown to contain 7-11 zinc atoms. These data suggest that the proteincontains repetitive zinc-binding domains. Analysis of the amino acid sequence reveals nine tandem similar units, each consisting of approximately 30 residues and containing two invariant pairs of cysteines and histidines, the most common ligands for zinc. The linear arrangement of these repeated, independently folding domains, each centred on a zinc ion, comprises the major part of the protein. Such a structure explains how this small protein can bind to the long internal control region of the 5S RNA gene, and stay bound during the passage of an RNA polymerase molecule.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:15:06