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Titolo:
Differentiation and quantitation of isomeric dipeptides by low-energy dissociation of copper(II)-bound complexes
Autore:
Tao, WA; Wu, LM; Cooks, RG;
Indirizzi:
Purdue Univ, Dept Chem, Aston Lab Mass Spectrometry, W Lafayette, IN 47907USA Purdue Univ W Lafayette IN USA 47907 ectrometry, W Lafayette, IN 47907USA
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
fascicolo: 5, volume: 12, anno: 2001,
pagine: 490 - 496
SICI:
1044-0305(200105)12:5<490:DAQOID>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-PHASE DISSOCIATION; PROTON-BOUND DIMERS; KINETIC METHOD; MASS-SPECTROMETRY; THERMOCHEMICAL DETERMINATIONS; AFFINITIES; PEPTIDES; ACIDS; IONS; PHENYLALKYLAMINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Cooks, RG Purdue Univ, Dept Chem, Aston Lab Mass Spectrometry, W Lafayette, IN 47907USA Purdue Univ W Lafayette IN USA 47907 , W Lafayette, IN 47907USA
Citazione:
W.A. Tao et al., "Differentiation and quantitation of isomeric dipeptides by low-energy dissociation of copper(II)-bound complexes", J AM SOC M, 12(5), 2001, pp. 490-496

Abstract

Application of the kinetic method based on the dissociation of transition metal centered cluster ions is extended from chiral analysis (Tao, W. A.; Zhang, D.; Nikolaev, E. N.; Cooks, R. G. J. Am. Chem. Soc. 2000, 122, 10598)to quantitative analysis of isomeric mixtures, including those with Leu/Ile substitutions. Copper(II)-bound complexes of pairs of peptide isomers aregenerated by electrospray ionization mass spectrometry and the trimeric complex [Cu-II(ref)(2)(A) - H](+) (analyte A, a mixture of isomeric peptides;reference compound ref, usually a peptide) is caused to undergo collisional dissociation. Competitive loss of the neutral reference compound or the neutral analyte yields two ionic products and the ratio of rates of the two competitive dissociations, viz. the product ion branching ratio R is shown to depend strongly on the regiochemistry of the analyte in the precursor [Cu-II(A)(ref)(2) - H](+) complex ion. Calibration curves are constructed by relating the branching ratio measured by the kinetic method, to the isomeric composition of the mixture to allow rapid quantitative isomer analysis. (J Am Soc Mass Spectrom 2001, 12, 490-496) (C) 2001 American Society for Mass Spectrometry.

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Documento generato il 12/07/20 alle ore 12:47:52