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Titolo:
Transendocardial delivery of autologous bone marrow enhances collateral perfusion and regional function in pigs with chronic experimental myocardial ischemia
Autore:
Fuchs, S; Baffour, R; Zhou, YF; Shou, M; Pierre, A; Tio, FO; Weissman, NJ; Leon, MB; Epstein, SE; Kornowski, R;
Indirizzi:
Washington Hosp Ctr, Cardiovasc Res Inst, Washington, DC 20010 USA Washington Hosp Ctr Washington DC USA 20010 nst, Washington, DC 20010 USA Biomed Res Fdn S Texas Inc, San Antonio, TX USA Biomed Res Fdn S Texas Inc San Antonio TX USA s Inc, San Antonio, TX USA Lenox Hill Hosp, Cardiovasc Res Fdn, New York, NY 10021 USA Lenox Hill Hosp New York NY USA 10021 asc Res Fdn, New York, NY 10021 USA
Titolo Testata:
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
fascicolo: 6, volume: 37, anno: 2001,
pagine: 1726 - 1732
SICI:
0735-1097(200105)37:6<1726:TDOABM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST-GROWTH-FACTOR; ENDOTHELIAL PROGENITOR CELLS; CORONARY-ARTERY OCCLUSION; BLOOD-FLOW; ELECTROMECHANICAL CHARACTERIZATION; GENE-TRANSFER; MODEL; HEART; ANGIOGENESIS; NEOVASCULARIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Fuchs, S Washington Hosp Ctr, Cardiovasc Res Inst, 110 Irving St NW,4B-1, Washington, DC 20010 USA Washington Hosp Ctr 110 Irving St NW,4B-1 Washington DC USA 20010
Citazione:
S. Fuchs et al., "Transendocardial delivery of autologous bone marrow enhances collateral perfusion and regional function in pigs with chronic experimental myocardial ischemia", J AM COL C, 37(6), 2001, pp. 1726-1732

Abstract

OBJECTIVES We tested the hypothesis that intramyocardial injection of autologous bone marrow (ABM) promotes collateral development in ischemic porcine myocardium. We also defined, in vitro, whether bone marrow (BM) cells secrete vascular endothelial growth factor (VEGF) and macrophage chemoattractant protein-1 (MCP-1). BACKGROUND The natural processes leading to collateral development are extremely complex, requiring multiple growth factors interacting in concert and in sequence. Because optimal angiogenesis may therefore, require multipleangiogenic factors, we thought that injection of BM, which contains cells that secrete numerous angiogenic factors, might provide optimal therapeuticangiogenesis,METHODS Bone marrow was cultured four weeks in vitro. Conditioned medium was assayed for VEGF and MCP-1 and was added to cultured pig aortic endothelial cells (PAEC) to assess proliferation. Four weeks after left circumflex ameroid implantation, freshly aspirated ABM (n = 7) or heparinized saline (n = 7) was injected transendocardially into the ischemic zone (0.2 ml/injection at 12 sites). Echocardiography to assess myocardial thickening and microspheres to assess perfusion were performed at rest and during stress. RESULTS Vascular endothelial growth factor and MCP-1 concentrations increased in a time-related manner. The conditioned medium enhanced, in a dose related manner, PAEC proliferation. Collateral flow (ischemic/normal zone X 100 improved in ABM-treated pigs (ABM: 98 +/- 14 vs. 83 +/- 12 at rest, p = 0.001; 89 +/- 18 vs. 78 +/- 12 during adenosine, p = 0.025; controls: 92 +/- 10 vs. 89 +/- 9 at rest, p = 0.49; 78 +/- 11 vs.77 +/- 5 during adenosine, p = 0.75). Similarly, contractility increased in ABM-treated pigs (ABM: 83 +/- 21 vs, 60 +/- 32 at rest, p = 0.04; 91 +/- 44 vs. 36 +/- 43 during pacing, p = 0.056; controls: 69 +/- 48 vs. 64 +/- 46 at rest, p = 0.74; 65 +/- 56 vs. 37 +/- 56 during pacing, p = 0.23). CONCLUSIONS Bone marrow cells secrete angiogenic factors that induce endothelial cell proliferation and, when injected transendocardially, augment collateral perfusion and myocardial function in ischemic myocardium. (J Am Coil Cardiol 2001;37:1726-32) (C) 2001 by the American College of Cardiology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:25:41