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Titolo:
Production of 7-deoxy-okadaic acid by a New Caledonian strain of Prorocentrum lima (Dinophyceae)
Autore:
Holmes, MJ; Lee, FC; Khoo, HW; Teo, SLM;
Indirizzi:
Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore Natl Univ Singapore Singapore Singapore 119260 ngapore 119260, Singapore Natl Univ Singapore, Trop Marine Sci Inst, Singapore 119223, Singapore Natl Univ Singapore Singapore Singapore 119223 ngapore 119223, Singapore
Titolo Testata:
JOURNAL OF PHYCOLOGY
fascicolo: 2, volume: 37, anno: 2001,
pagine: 280 - 288
SICI:
0022-3646(200104)37:2<280:PO7ABA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIARRHETIC SHELLFISH TOXINS; GAMBIERDISCUS-TOXICUS DINOPHYCEAE; CHROMATOGRAPHY MASS-SPECTROMETRY; CORAL-REEF ECOSYSTEM; OKADAIC ACID; LIQUID-CHROMATOGRAPHY; PROTEIN PHOSPHATASES; POISONING TOXINS; HERON-ISLAND; DINOFLAGELLATE;
Keywords:
7-deoxy-okadaic acid; diarrhetic shellfish poisoning; dinoflagellate; okadaic acid; Prorocentrum lima; toxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Holmes, MJ Natl Univ Singapore, Dept Biol Sci, Singapore 119260, SingaporeNatl Univ Singapore Singapore Singapore 119260 260, Singapore
Citazione:
M.J. Holmes et al., "Production of 7-deoxy-okadaic acid by a New Caledonian strain of Prorocentrum lima (Dinophyceae)", J PHYCOLOGY, 37(2), 2001, pp. 280-288

Abstract

7-Deoxy-okadaic acid and okadaic acid were identified as the major diarrhetic shellfish poisoning (DSP) toxins produced by a New Caledonian strain ofProrocentrum lima Ehrenberg, Dinophysistoxin-1 was not produced by this strain. The cellular concentrations of 7-deoxy-okadaic acid were about one tenth that of okadaic acid and were maximal (similar to1.4 pg.cell(-1)) during the stationary growth phase of batch culture. Autolytic hydrolysis of cell extracts did not increase the concentrations of 7-deoxy-okadaic acid, whereas okadaic acid production increased more than 4-fold, indicating that 7-deoxy-okadaic acid, unlike okadaic acid, is not directly derived from largesulfated precursors. 7-Deoxy-okadaic acid could be detected by liquid chromatogaphy-selected reaction monitoring mass spectrometry, HPLC-fluorescencedetection after derivatization with 9-anthryldiazomethane (ADAM), and inhibition of protein phosphatases. The solvent washes currently used for solid-phase clean-up of ADAM-derivatized DSP samples elute derivatized 7-deoxy-okadaic acid, indicating that the current sample clean-up protocol for HPLC-fluorescence detection would miss any contamination by this toxin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 19:59:05