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Titolo:
Caspase-3 activation in oligodendrocytes from the myelin-deficient rat
Autore:
Beesley, JS; Lavy, L; Eraydin, NB; Siman, R; Grinspan, JB;
Indirizzi:
Childrens Hosp Philadelphia, Dept Neurol Res, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Univ Penn, Dept Pharmacol, Philadelphia, PA USA Univ Penn Philadelphia PAUSA Penn, Dept Pharmacol, Philadelphia, PA USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 4, volume: 64, anno: 2001,
pagine: 371 - 379
SICI:
0360-4012(20010515)64:4<371:CAIOFT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PELIZAEUS-MERZBACHER-DISEASE; PROTEOLIPID PROTEIN GENE; GLIAL-CELL DEATH; POLY(ADP-RIBOSE) POLYMERASE; CULTURED OLIGODENDROCYTES; MONOCLONAL-ANTIBODY; DNA FRAGMENTATION; PLASMA-MEMBRANE; NERVOUS-SYSTEM; JIMPY MOUSE;
Keywords:
myelin-deficient rat; proteolipid protein; oligodendrocytes; apoptosis; caspases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Beesley, JS Abramson Res Ctr, Rm 514,3517 Civ Ctr Blvd, Philadelphia, PA 19104 USA Abramson Res Ctr Rm 514,3517 Civ Ctr Blvd Philadelphia PA USA 19104
Citazione:
J.S. Beesley et al., "Caspase-3 activation in oligodendrocytes from the myelin-deficient rat", J NEUROSC R, 64(4), 2001, pp. 371-379

Abstract

The myelin-deficient (MD) rat has a point mutation in its proteolipid protein (PLP) gene that causes severe dysmyelination and oligodendrocyte cell death. Using an in vitro model, we have shown that MD oligodendrocytes initially differentiate similarly to wild-type cells, expressing galactocerebroside, 2',3'-cyclic nucleotide 3'-phosphodiesterase, and myelin basic protein. However, at the time when PLP expression would normally begin, the MD oligodendrocytes die via an apoptotic pathway involving caspase activation. The active form of caspase-3 was detected, along with the cleavage products of poly-(ADP-ribose) polymerase (PARP) and spectrin, major targets of caspase-mediated proteolysis. A specific inhibitor of casapse-3, Ac-DEVD-CMK, reduced apoptosis in MD oligodendrocytes, but the rescued cells did not maturefully or express myelin-oligodendrocyte glycoprotein. These results suggest that mutant PLP affects not only cell death but also oligodendrocyte differentiation. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 11:39:52