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Titolo:
Temperature and time interval for culture of postmortem neurons from adultrat cortex
Autore:
Viel, JJ; McManus, DQ; Cady, C; Evans, MS; Brewer, GJ;
Indirizzi:
So Illinois Univ, Sch Med, Dept Med Microbiol & Immunol, Springfield, IL 62794 USA So Illinois Univ Springfield IL USA 62794 unol, Springfield, IL 62794 USA So Illinois Univ, Sch Med, Dept Neurol, Springfield, IL USA So Illinois Univ Springfield IL USA ed, Dept Neurol, Springfield, IL USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 4, volume: 64, anno: 2001,
pagine: 311 - 321
SICI:
0360-4012(20010515)64:4<311:TATIFC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BRAIN; HIPPOCAMPAL-NEURONS; CEREBRAL-ISCHEMIA; CARDIAC-ARREST; NERVOUS-SYSTEM; VISUAL-CORTEX; IN-VITRO; CELLS; HYPOTHERMIA; PROLIFERATION;
Keywords:
postmortem; neuron culture; ischemia; hypothermia; selective vulnerability; glutamate; neuron density;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Brewer, GJ So Illinois Univ, Sch Med, Dept Med Microbiol & Immunol, POB 19626, Springfield, IL 62794 USA So Illinois Univ POB 19626 Springfield IL USA 62794 L 62794 USA
Citazione:
J.J. Viel et al., "Temperature and time interval for culture of postmortem neurons from adultrat cortex", J NEUROSC R, 64(4), 2001, pp. 311-321

Abstract

For a model of neurological disease and ischemia, we extended recent work to culture adult postmortem rat brain neurons. Frontal cortex sections wereremoved from adult rats immediately following sacrifice and at different postmortem intervals and with the brain at either 22 degreesC or 4 degreesC,Brain could be stored four times longer at 4 degreesC between sacrifice and neuronal disaggregation to achieve the same 20% recovery of live cells from those plated compared to 22 degreesC, Each milligram of rat frontal cortex was estimated by the optical disector method to contain 160,000 neurons. When cells were isolated as rapidly as possible, 9% of the neurons originally present in the brain were viable. Various postmortem intervals from 2 to 24 hr resulted in a reduction from 6% to 3% of the cells originally present. After 5 days in culture, viable neurons were 23-42% of those isolated. Neuron-like cells that survived represented 40-75% of the viable cells, or 0.5-2.75% of those originally estimated to be present in the brain. Electrophysiology experiments show that cells isolated 0 and 24 hr postmortem had neuronal electrical properties, including an average resting membrane potential of -48 mV, voltage-sensitive currents, and action potentials. Neuron-like cells were immunoreactive for neuron-specific enolase, neurofilament 200, glutamate, MAP2, and tau after 2 weeks in culture. These experiments show that neuron-like cells can be reliably cultured from adult rat cortex up to 6 hr postmortem when stored at 22 degreesC and up to 24 hr postmortem when stored at 4 degreesC. These findings should encourage donation of human postmortem brain neurons for studies on ischemia, adult pharmacology, and neurological disease. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 06:34:58