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Titolo:
Analysis of the activity-deprived zebrafish mutant macho reveals an essential requirement of neuronal activity for the development of a fine-grained visuotopic map
Autore:
Gnuegge, L; Schmid, S; Neuhauss, SCF;
Indirizzi:
Max Planck Inst Entwicklungsbiol, D-72076 Tubingen, Germany Max Planck Inst Entwicklungsbiol Tubingen Germany D-72076 ingen, Germany Univ Tubingen, Inst Zool, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 t Zool, D-72076 Tubingen, Germany
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 10, volume: 21, anno: 2001,
pagine: 3542 - 3548
SICI:
0270-6474(20010515)21:10<3542:AOTAZM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOTECTAL PROJECTION; DANIO-RERIO; VISUAL-SYSTEM; OPTIC TECTUM; RANA-PIPIENS; GOLDFISH; GENES; AXONS; CONNECTIVITY; TETRODOTOXIN;
Keywords:
visual system; retinotectal projection; sodium currents; zebrafish; axon guidance; plasticity; Danio rerio; retinal ganglion cell; TTX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Neuhauss, SCF ETH Zurich, Dept Neuromorphol, Inst Brain Res, Winterthurerstr 190, CH-8057 Zurich, Switzerland ETH Zurich Winterthurerstr 190 Zurich Switzerland CH-8057 d
Citazione:
L. Gnuegge et al., "Analysis of the activity-deprived zebrafish mutant macho reveals an essential requirement of neuronal activity for the development of a fine-grained visuotopic map", J NEUROSC, 21(10), 2001, pp. 3542-3548

Abstract

The formation of a retinotopic map is thought to involve an activity- independent molecular phase for early steps of both axon pathfinding and projection and a later phase in which cross talk between retinal ganglion cells (RGCs) and tectal neurons modifies and refines the neuronal connections. We report that the maturation of the retinotopic map in the zebrafish tectum involves activity- dependent processes. Zebrafish larvae mutant for the genemacho (mao) lack neuronal activity in RGCs and also display an enlarged retinotectal projection field but no significant increase in single axon length. This morphological defect can be phenocopied by raising larvae under TTX- induced neural impulse blockade. The effect of activity deprivation is dependent on the developmental stage. The projection phenotype in mao as well as in the TTX- treated larvae develops between 4 and 6 d post- fertilization (dpf), after complete tectal coverage is first achieved. Electrophysiological recordings of RGCs in wild- type and mao zebrafish larvae reveal a temporally regulated reduction of sodium current in the mutant between 5 and6 dpf. This coincides with the time of the axonal projection shifting on the tectum to compensate for the disparate growth patterns of the retina andthe tectum. Our genetic and physiological analyses suggest a model in which neuronal activity in RGCs is needed for the establishment of morphological plasticity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 11:28:05