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Titolo:
Changes in preoptic and hypothalamic levels of progesterone receptor mRNA across the oestrous cycle of the ewe
Autore:
Scott, CJ; Pereira, AM; Tilbrook, AJ; Rawson, JA; Clarke, IJ;
Indirizzi:
Monash Univ, Dept Physiol, Clayton, Vic 3800, Australia Monash Univ Clayton Vic Australia 3800 siol, Clayton, Vic 3800, Australia Prince Henrys Inst Med Res, Clayton, Vic, Australia Prince Henrys Inst MedRes Clayton Vic Australia Clayton, Vic, Australia
Titolo Testata:
JOURNAL OF NEUROENDOCRINOLOGY
fascicolo: 5, volume: 13, anno: 2001,
pagine: 401 - 406
SICI:
0953-8194(200105)13:5<401:CIPAHL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; GONADOTROPIN-RELEASING-HORMONE; LUTEINIZING-HORMONE; ESTROUS-CYCLE; IMMUNOCYTOCHEMICAL LOCALIZATION; MONKEY HYPOTHALAMUS; OVARIECTOMIZED EWE; STEROID REGULATION; GUINEA-PIGS; RAT-BRAIN;
Keywords:
in situ hybridization; arcuate nucleus; ventromedial nucleus; oestrogen; gonadotropin releasing hormone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Scott, CJ Monash Univ, Dept Physiol, POB 13F, Clayton, Vic 3800, AustraliaMonash Univ POB 13F Clayton Vic Australia 3800 c 3800, Australia
Citazione:
C.J. Scott et al., "Changes in preoptic and hypothalamic levels of progesterone receptor mRNA across the oestrous cycle of the ewe", J NEUROENDO, 13(5), 2001, pp. 401-406

Abstract

We measured the levels of progesterone receptor (PR) mRNA in the hypothalamus and preoptic area (POA) of the ewe across the oestrous cycle. Perfusion-fixed hypothalamic tissue was collected from sheep killed during the luteal and follicular phases and during behavioural oestrus, Blood samples takenat the time of tissue collection verified that the oestrous ewes were undergoing a preovulatory luteinizing hormone (LH) surge, Matched sections weretaken from the POA, periventricular nucleus (PeVN), ventromedial nucleus (VMN) and arcuate nucleus of each animal, In situ hybridization was performed using a sheep specific, S-35-labelled riboprobe for PR and semiquantitative image analysis was conducted on emulsion-dipped slides. The number of silver grains per cell was greater in the VMN and arcuate nucleus of oestrousewes than in luteal and follicular phase ewes; there was no cyclic variation in the level of PR mRNA expression in the cells of the POA and PeVN. Thenumber of labelled cells per mm(2) in the VMN was higher in the oestrous ewes than in luteal phase and follicular phase ewes, The number of labelled cells in the PeVN was also higher in the oestrous ewes than in follicular phase ewes, but there was no cyclic variation in the POA and arcuate nucleus. In the ewe, the onset of behavioural oestrus corresponds to the onset of the preovulatory LH surge and increased PR mRNA expression at this time is likely to be due to the high concentrations of circulating oestrogen that precede this period.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 21:56:26