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Titolo:
The emergence of electron tomography as an important tool for investigating cellular ultrastructure
Autore:
McEwen, BF; Marko, M;
Indirizzi:
New York State Dept Hlth, Wadsworth Ctr Labs & Res, Div Mol Med, Albany, NY 12201 USA New York State Dept Hlth Albany NY USA 12201 ol Med, Albany, NY 12201 USA SUNY Albany, Dept Biomed Sci, Grad Sch Publ Hlth, Albany, NY 12201 USA SUNY Albany Albany NY USA 12201 Grad Sch Publ Hlth, Albany, NY 12201 USA
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 5, volume: 49, anno: 2001,
pagine: 553 - 563
SICI:
0022-1554(200105)49:5<553:TEOETA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSECT FLIGHT-MUSCLE; SPINDLE POLE BODY; GAMMA-TUBULIN; MICROSCOPIC TOMOGRAPHY; CRYOELECTRON TOMOGRAPHY; MICROTUBULE NUCLEATION; BIOLOGICAL STRUCTURES; IMAGE-RECONSTRUCTION; LOW-RESOLUTION; RING COMPLEX;
Keywords:
electron tomography; transmission electron microscopy; three-dimensional reconstruction; plastic embedding; cellular ultrastructure;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: McEwen, BF New York State Dept Hlth, Wadsworth Ctr Labs & Res, Div Mol Med, Pob 509, Albany, NY 12201 USA New York State Dept Hlth Pob 509 Albany NY USA 12201 12201 USA
Citazione:
B.F. McEwen e M. Marko, "The emergence of electron tomography as an important tool for investigating cellular ultrastructure", J HIST CYTO, 49(5), 2001, pp. 553-563

Abstract

Electron tomography has emerged as the leading method for the study of three-dimensional (3D) ultrastructure in the 5-20-nm resolution range. It is ideally suited for studying cell organelles, subcellular assemblies and, in some cases, whole cells. Tomography occupies a place in 3D biological electron microscopy between the work now being done at near-atomic resolution onisolated macromolecules or 2D protein arrays and traditional serial-section reconstructions of whole cells and tissue specimens. Tomography complements serial-section reconstruction by providing higher resolution in the depth dimension, whereas serial-section reconstruction is better able to trace continuity over long distances throughout the depth of a cell. The two techniques can be combined with good results for favorable specimens. Tomography also complements 3D macromolecular studies by offering sufficient resolution to locate the macromolecular complexes in their cellular context. The technology has matured to the point at which application of electron tomography to specimens in plastic sections is routine, and new developments to overcome limitations due to beam exposure and specimen geometry promise to further improve its capabilities. In this review we give a brief description of the methodology and a summary of the new insights gained in a few representative applications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 02:00:43