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Titolo:
Failure to down-regulate intragraft cytokine mRNA expression shortly afterclinical heart transplantation is associated with high incidence of acute rejection
Autore:
de Groot-Kruseman, HA; Baan, CC; Loonen, EHM; Mol, WM; Niesters, HGM; Maat, APWM; Balk, AHMM; Weimar, W;
Indirizzi:
Univ Rotterdam Hosp, Dept Internal Med Bd 299, NL-3015 GD Rotterdam, Netherlands Univ Rotterdam Hosp Rotterdam Netherlands NL-3015 GD terdam, Netherlands Univ Rotterdam Hosp, Diagnost Inst Mol Biol, NL-3015 GD Rotterdam, Netherlands Univ Rotterdam Hosp Rotterdam Netherlands NL-3015 GD terdam, Netherlands Univ Rotterdam Hosp, Dept Thorac Surg, NL-3015 GD Rotterdam, Netherlands Univ Rotterdam Hosp Rotterdam Netherlands NL-3015 GD terdam, Netherlands Univ Rotterdam Hosp, Dept Cardiol, NL-3015 GD Rotterdam, Netherlands Univ Rotterdam Hosp Rotterdam Netherlands NL-3015 GD terdam, Netherlands
Titolo Testata:
JOURNAL OF HEART AND LUNG TRANSPLANTATION
fascicolo: 5, volume: 20, anno: 2001,
pagine: 503 - 510
SICI:
1053-2498(200105)20:5<503:FTDICM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST-GROWTH-FACTOR; MONOCYTE CHEMOATTRACTANT PROTEIN-1; CANINE MYOCARDIUM; GENE-EXPRESSION; CYCLOSPORINE-A; T-CELLS; INDUCTION; DEXAMETHASONE; MODULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: de Groot-Kruseman, HA Univ Rotterdam Hosp, Dept Internal Med Bd 299, Dr Molewaterpl 40, NL-3015 GD Rotterdam, Netherlands Univ Rotterdam Hosp Dr Molewaterpl 40 Rotterdam Netherlands NL-3015 GD
Citazione:
H.A. de Groot-Kruseman et al., "Failure to down-regulate intragraft cytokine mRNA expression shortly afterclinical heart transplantation is associated with high incidence of acute rejection", J HEART LUN, 20(5), 2001, pp. 503-510

Abstract

Background: Brain-death, ischemia and reperfusion damage have been implicated as initial factors that lead to a cascade of immunologic events that result in allograft rejection in experimental animals. Cytokines are thought to play a central role in this process. Therefore,, we evaluated intragraftcytokine mRNA expression at an early stage after clinical heart transplantation and related these data to ischemia, immunosuppression, and rejection. Methods: We sampled endomyocardial biopsies at 30 minutes (EMB 0) and at 1week (EMB 1) after transplantation from 20 cardiac allograft recipients. Intragraft monocyte chemoattractant protein (MCP-1) and basic fibroblast growth factor (bFGF) mRNA expression levels were quantitatively measured usingcompetitive template Reverse-transcriptase polymerase chain reaction (RT-PCR). Results: We measured significantly lower MCP-1 and bFGF mRNA expression levels in EMB 1 compared with EMB 0 (MCP-1, p = 0.006; bFGF, p = 0.019). We found no direct correlation between the cytokine mRNA expression levels in EMB 0 or EMB 1 and ischemic times, induction therapy, or cyclosporine whole-blood trough levels. Patients with a high incidence of acute rejection episodes (>2 in the first year) had higher bFGF mRNA expression levels (p = 0.009) and comparable MCP-1 mRNA expression levels (p = 0.378) at 1 week, compared with patients with a lower rejection incidence. The MCP-1 and bFGF mRNA expression levels in the first week were not associated with the development of graft vascular disease in the first year post-transplant. Conclusions: We found a significant decrease of intragraft MCP-1 and bFGF mRNA expression levels in the first post-operative week. Patients with a high incidence of acute rejection had higher bFGF mRNA expression levels in their first week biopsy. Therefore, we conclude that patients who fail to down-regulate their bFGF mRNA expression early after transplantation are at higher risk for acute rejection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:12:31