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Titolo:
Determination of a new oral iron chelator, ICL670, and its iron complex inplasma by high-performance liquid chromatography and ultraviolet detection
Autore:
Rouan, MC; Marfil, F; Mangoni, P; Sechaud, R; Humbert, H; Maurer, G;
Indirizzi:
Novartis Pharma SA, Drug Metab & Pharmacokinet, F-92500 Rueil Malmaison, France Novartis Pharma SA Rueil Malmaison France F-92500 ueil Malmaison, France Novartis Pharma AG, Clin Pharmacol, CH-4002 Basel, Switzerland Novartis Pharma AG Basel Switzerland CH-4002 CH-4002 Basel, Switzerland
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 203 - 213
SICI:
1387-2273(20010505)755:1-2<203:DOANOI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFERRIN-BOUND IRON; BIOLOGICAL-FLUIDS; QUANTIFICATION; DESFERRIOXAMINE; DEFEROXAMINE; FERRIOXAMINE; DEFERIPRONE; METABOLITES; EFFICACY; KINETICS;
Keywords:
ICL670; iron chelators;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Rouan, MC Novartis Pharma SA, Drug Metab & Pharmacokinet, 2-4 Rue Lionel Terray, F-92500 Rueil Malmaison, France Novartis Pharma SA 2-4 Rue Lionel Terray Rueil Malmaison France F-92500
Citazione:
M.C. Rouan et al., "Determination of a new oral iron chelator, ICL670, and its iron complex inplasma by high-performance liquid chromatography and ultraviolet detection", J CHROMAT B, 755(1-2), 2001, pp. 203-213

Abstract

ICL670 is a representative of a new class of orally active tridentate selective iron chelators. Two molecules of ICL670 are required to form a complete hexacoordinate chelate Fe-[ICL670](2) with one ferric iron. A simple andrapid HPLC-UV method for the separate determination of ICL670 and Fe-[ICL670](2) in the plasma of iron-overloaded patients is described. Plasma samples were prepared as rapidly as possible, the tubes being kept at 4 degreesC. Plasma proteins were precipitated with methanol. The supernatant was diluted with water and placed on the refrigerated sample rack of an autosamplerbefore injection. The chromatographic separations were achieved on an Alltima C-18 column using 0.05 M Na2HPO4 and 0.01 M tetrabutylammonium hydrogensulfate-acetonitrile-methanol (41:9:50, v/v/v) as mobile phase. The analytes were detected at 295 nm. Calibration and quality control samples were prepared in normal human plasma. The mean accuracy (n=6) over the entire investigated concentration range 0.25-20 mug/ml ranged from 91 to 109% with a coefficient of variation (C.V.) from 4 to 8% for ICL670, and from 95 to 105%with a C.V. from 2 to 20% for the iron complex. The dissociation of the complex during analysis was shown to be marginal. The iron removal from plasma of iron-overloaded patients by free ICL670 during analysis was low. The in vitro iron transfer from the iron pools of iron-overloaded plasma onto ICL670 was shown to be a slow process. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:39:56