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Titolo:
High-performance liquid chromatographic determination of deoxycytidine monophosphate and methyldeoxycytidine monophosphate for DNA demethylation monitoring: experimental design and artificial neural networks optimisation
Autore:
Havlis, J; Madden, JE; Revilla, AL; Havel, J;
Indirizzi:
Masaryk Univ, Fac Sci, Dept Analyt Chem, CS-61137 Brno, Czech Republic Masaryk Univ Brno Czech Republic CS-61137 CS-61137 Brno, Czech Republic
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 185 - 194
SICI:
1387-2273(20010505)755:1-2<185:HLCDOD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY-ZONE-ELECTROPHORESIS; METHYLATION; PREDICTION; GENE; COMBINATION;
Keywords:
artificial neural networks; deoxycytidine monophosphate; methyldeoxycytidine monophosphate; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Havlis, J Masaryk Univ, Fac Sci, Dept Analyt Chem, Kotlarska 2, CS-61137 Brno, CzechRepublic Masaryk Univ Kotlarska 2 Brno Czech Republic CS-61137 hRepublic
Citazione:
J. Havlis et al., "High-performance liquid chromatographic determination of deoxycytidine monophosphate and methyldeoxycytidine monophosphate for DNA demethylation monitoring: experimental design and artificial neural networks optimisation", J CHROMAT B, 755(1-2), 2001, pp. 185-194

Abstract

The optimal conditions for the separation of 2'-deoxycytidine-5'-monophosphate and 5-methyl-2'-deoxycytidine-5'-monophosphate in the matrix of other natural occurring nucleotides after digest of DNA were investigated. Using experimental design combined with artificial neural networks, efficient optimisation of the HPLC separation conditions was performed. The mobile phasecomposition was optimised on the basis of its three components (concentration of phosphate, content of methanol and pH), The best separation was obtained with a mobile phase containing 50 mM phosphate, pH 5.5 and 6% methanol, The final resolution achieved between 2'-deoxycytidine-5'-monophosphate and 5-methyl-2'-deoxy cytidine-5'-monophosphate was equal to 2.78. Finally, the optimised system was successfully tested on the nucleotide mixture solution to determine the methylation state of 2'-deoxycytidine-5'-monophosphate in DNA in the search for FMR1 gene changes. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:29:52