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Titolo:
Sensitive and specific determination of clindamycin in human serum and bone tissue applying liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry
Autore:
Martens-Lobenhoffer, J; Banditt, P;
Indirizzi:
Univ Magdeburg, Univ Klinikum, Inst Klin Pharmakol, D-39120 Magdeburg, Germany Univ Magdeburg Magdeburg Germany D-39120 kol, D-39120 Magdeburg, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 143 - 149
SICI:
1387-2273(20010505)755:1-2<143:SASDOC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PLASMA;
Keywords:
clindamycin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Martens-Lobenhoffer, J Univ Magdeburg, Univ Klinikum, Inst Klin Pharmakol,Leipziger Str 44, D-39120 Magdeburg, Germany Univ Magdeburg Leipziger Str 44 Magdeburg Germany D-39120
Citazione:
J. Martens-Lobenhoffer e P. Banditt, "Sensitive and specific determination of clindamycin in human serum and bone tissue applying liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry", J CHROMAT B, 755(1-2), 2001, pp. 143-149

Abstract

A method for the quantification of clindamycin in human serum and in humanbone tissue samples applying high-performance liquid chromatography with atmospheric pressure chemical ionization-mass spectrometry (APCI-MS) is presented, Lincomycin is used as the internal standard. Serum samples are prepared only by protein precipitation with acetonitrile. Bone tissue samples have to be crushed and homogenized in extraction buffer prior to analysis. The chromatographic separation is achieved on an RP-18 stationary phase with 0.02% trifluoroacetic acid in water 60%/ acetonitrile 40% v/v as mobile phase. The limits of quantification are 0.1 mug/ml for serum samples and 0.1 mug/g for bone tissue samples. The coefficients of variation for the assays are 4.48 and 8.41% at the limit of quantification for serum and bone tissuesamples, respectively. Bone tissue samples as small as 50 mg can be used, (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 07:08:16