Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Stir bar sorptive extraction-thermal desorption-capillary gas chromatography-mass spectrometry applied to the analysis of polychlorinated biphenyls in human sperm
Autore:
Benijts, T; Vercammen, J; Dams, R; Tuan, HP; Lambert, W; Sandra, P;
Indirizzi:
State Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium State Univ GhentGhent Belgium B-9000 Organ Chem, B-9000 Ghent, Belgium State Univ Ghent, Toxicol Lab, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 Toxicol Lab, B-9000 Ghent, Belgium
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 137 - 142
SICI:
1387-2273(20010505)755:1-2<137:SBSEDG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADIPOSE-TISSUE; HUMAN-MILK; SAMPLES; ORGANOCHLORINE; CONTAMINANTS; POLLUTANTS; CONGENERS; TUBES; SERUM; PCBS;
Keywords:
stir bar sorptive extraction; polychlorinated biphenyls;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Sandra, P State Univ Ghent, Dept Organ Chem, Krijgslaan 281, B-9000 Ghent,Belgium State Univ Ghent Krijgslaan 281 Ghent Belgium B-9000 t, Belgium
Citazione:
T. Benijts et al., "Stir bar sorptive extraction-thermal desorption-capillary gas chromatography-mass spectrometry applied to the analysis of polychlorinated biphenyls in human sperm", J CHROMAT B, 755(1-2), 2001, pp. 137-142

Abstract

Stir bar sorptive extraction (SBSE) on polydimethylsiloxane (PDMS) was applied to the enrichment of polychlorinated biphenyls (PCBs) from human sperm. The seven Ballschmiter PCBs were used as model compounds. The extracted PCBs were then thermally desorbed from the stir bar and analysed on-line by capillary gas chromatography (CGC) with mass spectrometric detection (MS). Method development started with the analysis of PCBs spiked in water. Methanol had to be added to the samples in order to reduce the influence of glass adsorption on recovery and reproducibility. Recoveries in water for all PCSs varied around 50-60% and were limited for low molecular mass (MM) PCBs by polarity changes in the sample due to methanol addition and for high MM PCBs by non-equilibrium conditions. Matrix suppression by the lipophilic medium lowered the recoveries in the sperm samples proportional with PCB polarity. The method was validated and although limits of detection (LOD) for the individual congeners were in the sub-ppt level (<pg/ml), the limit of quantification (LOQ) was set at 10 ppt (10 pg/ml). (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 18:28:23