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Titolo:
Lamotrigine analysis in blood and brain by high-performance liquid chromatography
Autore:
Castel-Branco, MM; Almeida, AM; Falcao, AC; Macedo, TA; Caramona, MM; Lopez, FG;
Indirizzi:
Univ Coimbra, Fac Pharm, Pharmacol Lab, P-3000295 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3000295 Lab, P-3000295 Coimbra, Portugal Univ Coimbra, Fac Med, Inst Expt Pharmacol & Therapeut, P-3004504 Coimbra,Portugal Univ Coimbra Coimbra Portugal P-3004504 peut, P-3004504 Coimbra,Portugal Univ Salamanca, Fac Pharm, Dept Pharmaceut Technol, Salamanca 37007, SpainUniv Salamanca Salamanca Spain 37007 eut Technol, Salamanca 37007, Spain
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 119 - 127
SICI:
1387-2273(20010505)755:1-2<119:LAIBAB>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; IN-VIVO MICRODIALYSIS; HUMAN PLASMA; ANTIEPILEPTIC DRUG; METHODS VALIDATION; HUMAN SERUM; RAT-BRAIN; ASSAY; CARBAMAZEPINE; PHENYTOIN;
Keywords:
lamotrigine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Falcao, AC Univ Coimbra, Fac Pharm, Pharmacol Lab, P-3000295 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3000295 295 Coimbra, Portugal
Citazione:
M.M. Castel-Branco et al., "Lamotrigine analysis in blood and brain by high-performance liquid chromatography", J CHROMAT B, 755(1-2), 2001, pp. 119-127

Abstract

A reversed-phase high-performance liquid chromatography assay was developed and validated to determine plasma and brain lamotrigine concentrations allowing pharmacokinetic-pharmacodynamic studies of this new antiepileptic drug in patients and laboratory animals. Lamotrigine and its internal standard were extracted, under alkaline conditions, from plasma and brain homogenate, into ethyl acetate; brain proteins were previously precipitated with trichloroacetic acid. The method was linear between 0.1 and 15.0 mg/l for plasma, with a detection limit of 0.008 mg/l, and between 0.1 and 5.0 mg/l forbrain homogenate, with a detection limit of 0.023 mg/l. The method proved to be simple, useful and appropriate, not only for clinical and experimental research, but also for routine monitoring of lamotrigine concentrations in patients. (C) 2001 Elsevier Science B.V, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:52:44